Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

Mads Delbo Larsen; Rafael Bayarri-Olmos; Peter Garred; Lars Hviid

doi:https://doi.org/10.1007/978-1-0716-2189-9_50

Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

Mads Delbo Larsen, Rafael Bayarri-Olmos, Peter Garred, Lars Hviid

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

1 Citation (Scopus)

Abstract

Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	673-678
Number of pages	6
Volume	2470
DOIs	https://doi.org/10.1007/978-1-0716-2189-9_50
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2470

Keywords

Antibodies
Complement
ELISA
Malaria
PfEMP1

Access to Document

https://doi.org/10.1007/978-1-0716-2189-9_50

Cite this

Larsen, M. D., Bayarri-Olmos, R., Garred, P., & Hviid, L. (2022). Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies. In Methods in Molecular Biology (Vol. 2470, pp. 673-678). (Methods in Molecular Biology; Vol. 2470). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-2189-9_50

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abstract = "Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.",

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Larsen, MD, Bayarri-Olmos, R, Garred, P & Hviid, L 2022, Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies. in Methods in Molecular Biology. vol. 2470, Methods in Molecular Biology, vol. 2470, Humana Press Inc., pp. 673-678. https://doi.org/10.1007/978-1-0716-2189-9_50

Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies. / Larsen, Mads Delbo; Bayarri-Olmos, Rafael; Garred, Peter et al.

Methods in Molecular Biology. Vol. 2470 Humana Press Inc., 2022. p. 673-678 (Methods in Molecular Biology; Vol. 2470).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

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AU - Bayarri-Olmos, Rafael

AU - Garred, Peter

AU - Hviid, Lars

PY - 2022

Y1 - 2022

N2 - Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

AB - Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.

KW - Antibodies

KW - Complement

KW - ELISA

KW - Malaria

KW - PfEMP1

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DO - https://doi.org/10.1007/978-1-0716-2189-9_50

M3 - Chapter

C2 - 35881382

VL - 2470

T3 - Methods in Molecular Biology

SP - 673

EP - 678

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Larsen MD, Bayarri-Olmos R, Garred P, Hviid L. Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies. In Methods in Molecular Biology. Vol. 2470. Humana Press Inc. 2022. p. 673-678. (Methods in Molecular Biology). doi: https://doi.org/10.1007/978-1-0716-2189-9_50

Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies

Abstract

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