TY - JOUR
T1 - Establishment of the european meningococcal strain collection genome library (EMSC-GL) for the 2011 to 2012 epidemiological year
AU - Bratcher, Holly B.
AU - Brehony, Carina
AU - Heuberger, Sigrid
AU - Pieridou-Bagatzouni, Despo
AU - Křížová, Pavla
AU - Hoffmann, Steen
AU - Toropainen, Maija
AU - Taha, Muhamed-Kheir
AU - Claus, Heike
AU - Tzanakaki, Georgina
AU - Erdôsi, T. mea
AU - Galajeva, Jelena
AU - van der Ende, Arie
AU - Skoczyńska, Anna
AU - Pana, Marina
AU - Vaculíková, Alena
AU - Paragi, Metka
AU - Maiden, Martin C. J.
AU - Caugant, Dominique A.
PY - 2018
Y1 - 2018
N2 - Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011–June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neis-seria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n = 525; 65.7%) or C (n = 163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n = 485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n = 64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n = 204) of isolates contained alleles encoding the fHbp and/or the PorA VR1 vaccine component, but most genomes (n = 513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated geno-types in Europe, enabling the monitoring of hyperinva-sive lineages, outbreak identification, and supporting vaccine programme implementation.
AB - Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011–June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neis-seria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n = 525; 65.7%) or C (n = 163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n = 485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n = 64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n = 204) of isolates contained alleles encoding the fHbp and/or the PorA VR1 vaccine component, but most genomes (n = 513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated geno-types in Europe, enabling the monitoring of hyperinva-sive lineages, outbreak identification, and supporting vaccine programme implementation.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85047934775&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/29790460
U2 - https://doi.org/10.2807/1560-7917.ES.2018.23.20.17-00474
DO - https://doi.org/10.2807/1560-7917.ES.2018.23.20.17-00474
M3 - Article
C2 - 29790460
SN - 1025-496X
VL - 23
JO - Euro surveillance
JF - Euro surveillance
IS - 20
M1 - 17-00474
ER -