Expression of the IL-6 gene induces differentiation of a human monoclonal EBV-transformed B cell line

G. J. Jochems; R. J. Bende; M. R. Klein; W. P. Zeijlemaker; R. A. van Lier

Expression of the IL-6 gene induces differentiation of a human monoclonal EBV-transformed B cell line

G. J. Jochems, R. J. Bende, M. R. Klein, W. P. Zeijlemaker, R. A. van Lier

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To study the role of interleukin (IL)-6 as a growth and differentiation factor for Epstein-Barr virus (EBV)-transformed B lymphocytes, we transfected the cDNA coding for human IL-6 in a monoclonal IgG1-secreting EBV B cell line. Two independent clones were selected that constitutively secreted high amounts of IL-6. These clones showed enhanced levels of IL-6 and tumor necrosis factor alpha secretion when compared to non-IL-6 transfected controls. Moreover, they could efficiently be recovered from low cell density cultures in limiting dilutions when plated on a feeder layer of heterologous EBV B cells. IL-6-induced phenotypical changes comprised a significant rise in immunoglobulin secretion levels and enhanced membrane expression of CD25 (the beta chain of the IL-2 receptor) and of the B cell differentiation antigen CD40. IL-6-dependent down modulation of CD38 and of the adhesion structure VLA4 were also observed. Our data support the notion that IL-6 can serve as an growth and differentiation factor for EBV B cells

Original language	English
Pages (from-to)	124-133
Journal	Human antibodies and hybridomas
Volume	4
Issue number	3
Publication status	Published - 1993

Cite this

@article{d675ec2499db4155a573f1d48b5da163,

title = "Expression of the IL-6 gene induces differentiation of a human monoclonal EBV-transformed B cell line",

abstract = "To study the role of interleukin (IL)-6 as a growth and differentiation factor for Epstein-Barr virus (EBV)-transformed B lymphocytes, we transfected the cDNA coding for human IL-6 in a monoclonal IgG1-secreting EBV B cell line. Two independent clones were selected that constitutively secreted high amounts of IL-6. These clones showed enhanced levels of IL-6 and tumor necrosis factor alpha secretion when compared to non-IL-6 transfected controls. Moreover, they could efficiently be recovered from low cell density cultures in limiting dilutions when plated on a feeder layer of heterologous EBV B cells. IL-6-induced phenotypical changes comprised a significant rise in immunoglobulin secretion levels and enhanced membrane expression of CD25 (the beta chain of the IL-2 receptor) and of the B cell differentiation antigen CD40. IL-6-dependent down modulation of CD38 and of the adhesion structure VLA4 were also observed. Our data support the notion that IL-6 can serve as an growth and differentiation factor for EBV B cells",

author = "Jochems, {G. J.} and Bende, {R. J.} and Klein, {M. R.} and Zeijlemaker, {W. P.} and {van Lier}, {R. A.}",

year = "1993",

language = "English",

volume = "4",

pages = "124--133",

journal = "Human antibodies and hybridomas",

issn = "0956-960X",

publisher = "Butterworth Scientific Ltd.",

number = "3",

}

TY - JOUR

T1 - Expression of the IL-6 gene induces differentiation of a human monoclonal EBV-transformed B cell line

AU - Jochems, G. J.

AU - Bende, R. J.

AU - Klein, M. R.

AU - Zeijlemaker, W. P.

AU - van Lier, R. A.

PY - 1993

Y1 - 1993

N2 - To study the role of interleukin (IL)-6 as a growth and differentiation factor for Epstein-Barr virus (EBV)-transformed B lymphocytes, we transfected the cDNA coding for human IL-6 in a monoclonal IgG1-secreting EBV B cell line. Two independent clones were selected that constitutively secreted high amounts of IL-6. These clones showed enhanced levels of IL-6 and tumor necrosis factor alpha secretion when compared to non-IL-6 transfected controls. Moreover, they could efficiently be recovered from low cell density cultures in limiting dilutions when plated on a feeder layer of heterologous EBV B cells. IL-6-induced phenotypical changes comprised a significant rise in immunoglobulin secretion levels and enhanced membrane expression of CD25 (the beta chain of the IL-2 receptor) and of the B cell differentiation antigen CD40. IL-6-dependent down modulation of CD38 and of the adhesion structure VLA4 were also observed. Our data support the notion that IL-6 can serve as an growth and differentiation factor for EBV B cells

AB - To study the role of interleukin (IL)-6 as a growth and differentiation factor for Epstein-Barr virus (EBV)-transformed B lymphocytes, we transfected the cDNA coding for human IL-6 in a monoclonal IgG1-secreting EBV B cell line. Two independent clones were selected that constitutively secreted high amounts of IL-6. These clones showed enhanced levels of IL-6 and tumor necrosis factor alpha secretion when compared to non-IL-6 transfected controls. Moreover, they could efficiently be recovered from low cell density cultures in limiting dilutions when plated on a feeder layer of heterologous EBV B cells. IL-6-induced phenotypical changes comprised a significant rise in immunoglobulin secretion levels and enhanced membrane expression of CD25 (the beta chain of the IL-2 receptor) and of the B cell differentiation antigen CD40. IL-6-dependent down modulation of CD38 and of the adhesion structure VLA4 were also observed. Our data support the notion that IL-6 can serve as an growth and differentiation factor for EBV B cells

M3 - Article

C2 - 8395233

SN - 0956-960X

VL - 4

SP - 124

EP - 133

JO - Human antibodies and hybridomas

JF - Human antibodies and hybridomas

IS - 3

ER -