TY - JOUR
T1 - Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice
AU - van der Wijk, Anne-Eva
AU - Wisniewska-Kruk, Joanna
AU - Vogels, Ilse M. C.
AU - van Veen, Henk A.
AU - Ip, Wing Fung
AU - van der Wel, Nicole N.
AU - van Noorden, Cornelis J. F.
AU - Schlingemann, Reinier O.
AU - Klaassen, Ingeborg
PY - 2019/4
Y1 - 2019/4
N2 - Insight into the molecular and cellular processes in blood-retinal barrier (BRB) development, including the contribution of paracellular and transcellular pathways, is still incomplete but may help to understand the inverse process of BRB loss in pathologic eye conditions. In this comprehensive observational study, we describe in detail the formation of the BRB at the molecular level in physiologic conditions, using mice from postnatal day (P)3 to P25. Our data indicate that immature blood vessels already have tight junctions at P5, before the formation of a functional BRB. Expression of the endothelial cell-specific protein plasmalemma vesicle-associated protein (PLVAP), which is known to be involved in transcellular transport and associated with BRB permeability, decreased during development and was absent when a functional barrier was formed. Moreover, we show that PLVAP deficiency causes a transient delay in retinal vascular development and changes in mRNA expression levels of endothelial permeability pathway proteins.-Van der Wijk, A.-E., Wisniewska-Kruk, J., Vogels, I. M. C., van Veen, H. A., Ip, W. F., van der Wel, N. N., van Noorden, C. J. F., Schlingemann, R. O., Klaassen, I. Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice.
AB - Insight into the molecular and cellular processes in blood-retinal barrier (BRB) development, including the contribution of paracellular and transcellular pathways, is still incomplete but may help to understand the inverse process of BRB loss in pathologic eye conditions. In this comprehensive observational study, we describe in detail the formation of the BRB at the molecular level in physiologic conditions, using mice from postnatal day (P)3 to P25. Our data indicate that immature blood vessels already have tight junctions at P5, before the formation of a functional BRB. Expression of the endothelial cell-specific protein plasmalemma vesicle-associated protein (PLVAP), which is known to be involved in transcellular transport and associated with BRB permeability, decreased during development and was absent when a functional barrier was formed. Moreover, we show that PLVAP deficiency causes a transient delay in retinal vascular development and changes in mRNA expression levels of endothelial permeability pathway proteins.-Van der Wijk, A.-E., Wisniewska-Kruk, J., Vogels, I. M. C., van Veen, H. A., Ip, W. F., van der Wel, N. N., van Noorden, C. J. F., Schlingemann, R. O., Klaassen, I. Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice.
KW - Tight junctions
KW - Transcellular permeability
KW - VEGF signaling
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064114992&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30698992
U2 - https://doi.org/10.1096/fj.201801499RRR
DO - https://doi.org/10.1096/fj.201801499RRR
M3 - Article
C2 - 30698992
SN - 0892-6638
VL - 33
SP - 5320
EP - 5333
JO - FASEB Journal
JF - FASEB Journal
IS - 4
ER -