Extracellular vesicles in human follicular fluid do not promote coagulation

Cordula Franz; Anita N. Böing; Markus Montag; Thomas Strowitzki; Udo R. Markert; Sebastiaan Mastenbroek; Rienk Nieuwland; Bettina Toth

doi:https://doi.org/10.1016/j.rbmo.2016.08.005

Extracellular vesicles in human follicular fluid do not promote coagulation

Cordula Franz, Anita N. Böing, Markus Montag, Thomas Strowitzki, Udo R. Markert, Sebastiaan Mastenbroek, Rienk Nieuwland, Bettina Toth

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

Abstract

Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles

Original language	English
Pages (from-to)	652-655
Journal	Reproductive BioMedicine Online
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/j.rbmo.2016.08.005
Publication status	Published - 2016

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https://doi.org/10.1016/j.rbmo.2016.08.005

Cite this

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title = "Extracellular vesicles in human follicular fluid do not promote coagulation",

abstract = "Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles",

author = "Cordula Franz and B{\"o}ing, {Anita N.} and Markus Montag and Thomas Strowitzki and Markert, {Udo R.} and Sebastiaan Mastenbroek and Rienk Nieuwland and Bettina Toth",

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journal = "Reproductive BioMedicine Online",

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T1 - Extracellular vesicles in human follicular fluid do not promote coagulation

AU - Franz, Cordula

AU - Böing, Anita N.

AU - Montag, Markus

AU - Strowitzki, Thomas

AU - Markert, Udo R.

AU - Mastenbroek, Sebastiaan

AU - Nieuwland, Rienk

AU - Toth, Bettina

PY - 2016

Y1 - 2016

N2 - Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles

AB - Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles

U2 - https://doi.org/10.1016/j.rbmo.2016.08.005

DO - https://doi.org/10.1016/j.rbmo.2016.08.005

M3 - Article

C2 - 27569704

SN - 1472-6483

VL - 33

SP - 652

EP - 655

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

IS - 5

ER -