TY - JOUR
T1 - Extravasation of Microspheres in a Rat Model of Silent Brain Infarcts
AU - van der Wijk, Anne-Eva
AU - Lachkar, Nadia
AU - de Vos, Judith
AU - Grootemaat, Anita E.
AU - van der Wel, Nicole N.
AU - Hordijk, Peter L.
AU - Bakker, Erik N. T. P.
AU - vanBavel, Ed
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background and Purpose- We developed a rat model of silent brain infarcts based on microsphere infusion and investigated their impact on perfusion and tissue damage. Second, we studied the extent and mechanisms of perfusion recovery. Methods- At day 0, 15 µm fluorescent microspheres were injected into the right common carotid artery of F344 rats. At days 1, 7, or 28, the brain was removed, cut in 100-µm cryosections, and processed for immunofluorescent staining and analysis. Results- Injection of microspheres caused mild and transient damage to the treated hemisphere, with a decrease in perfused capillary volume at day 1, as compared with the untreated hemisphere. At day 1 but not at days 7 and 28, we observed IgG staining outside of the vessels, indicating vessel leakage. All microspheres were located inside the lumen of the vessels at day 1, whereas the vast majority (≈80%) of the microspheres were extravascular at day 7, and 100% at day 28. This was accompanied by restoration of perfused capillary volume. Conclusions- Microspheres cause mild and transient damage, and effective extravasation mechanisms exist in the brain to clear microsized emboli from the vessels.
AB - Background and Purpose- We developed a rat model of silent brain infarcts based on microsphere infusion and investigated their impact on perfusion and tissue damage. Second, we studied the extent and mechanisms of perfusion recovery. Methods- At day 0, 15 µm fluorescent microspheres were injected into the right common carotid artery of F344 rats. At days 1, 7, or 28, the brain was removed, cut in 100-µm cryosections, and processed for immunofluorescent staining and analysis. Results- Injection of microspheres caused mild and transient damage to the treated hemisphere, with a decrease in perfused capillary volume at day 1, as compared with the untreated hemisphere. At day 1 but not at days 7 and 28, we observed IgG staining outside of the vessels, indicating vessel leakage. All microspheres were located inside the lumen of the vessels at day 1, whereas the vast majority (≈80%) of the microspheres were extravascular at day 7, and 100% at day 28. This was accompanied by restoration of perfused capillary volume. Conclusions- Microspheres cause mild and transient damage, and effective extravasation mechanisms exist in the brain to clear microsized emboli from the vessels.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85067303386&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31136287
U2 - https://doi.org/10.1161/STROKEAHA.119.024975
DO - https://doi.org/10.1161/STROKEAHA.119.024975
M3 - Article
C2 - 31136287
SN - 0039-2499
VL - 50
SP - 1590
EP - 1594
JO - Stroke
JF - Stroke
IS - 6
ER -