@article{fe3b9dcf10f4474bad88b35ff0e40b23,
title = "Tandem mass tag-based thermal proteome profiling for the discovery of drug-protein interactions in cancer cells",
abstract = "Identification of effector targets is imperative to the characterization of the mechanisms of action of novel small molecules. Here, we describe steps to identify effector drug-protein interactions in lysates derived from cancer cell lines using a thermal proteome profiling (TPP) protocol. Building on existing TTP approaches, we detail the use of an in-solution trypsin digestion technique to streamline sample preparation, a nonparametric analysis to rank proteins for prioritization, and a follow-up strategy for identifying effector interactors. For complete details on the use and execution of this protocol, please refer to Johnson et al. (2022).",
keywords = "Cancer, Cell Biology, Mass Spectrometry, Molecular Biology, Protein Biochemistry, Proteomics",
author = "Johnson, {Fraser D.} and Hughes, {Christopher S.} and Alvin Liu and Lockwood, {William W.} and Morin, {Gregg B.}",
note = "Funding Information: We acknowledge the original work of Mikhail M. Savitski and colleagues in developing the TPP method, from which this work is derived. This work was funded by the BC Cancer Foundation, the Cancer Research Society, Canadian Institutes of Health Research (CIHR; PJT-148725), and the Terry Fox Research Institute. Methodology, F.D.J. A.L. C.S.H. W.W.L. G.B.M.; Formal Analysis, F.D.J. A.L. W.W.L. G.B.M.; Visualization, A.L. C.S.H. W.W.L.; Writing—Original Draft, F.D.J. C.S.H. W.W.L. G.B.M.; Writing—Review and editing, F.D.J. C.S.H. W.W.L. G.B.M.; Resources, W.W.L.; Supervision, W.W.L. G.B.M. W.W.L. is a consultant of HyperBio Therapeutics. Funding Information: We acknowledge the original work of Mikhail M. Savitski and colleagues in developing the TPP method, from which this work is derived. This work was funded by the BC Cancer Foundation , the Cancer Research Society , Canadian Institutes of Health Research (CIHR; PJT-148725 ), and the Terry Fox Research Institute. Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
month = mar,
day = "17",
doi = "https://doi.org/10.1016/j.xpro.2022.102012",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",
}