TY - JOUR
T1 - Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity
AU - Sprokholt, Joris K.
AU - Hertoghs, Nina
AU - Geijtenbeek, Teunis B. H.
PY - 2016
Y1 - 2016
N2 - In this chapter we describe a fluorescent bead-binding assay, which is an efficient and feasible method to measure interaction between ligands and receptors on cells. In principle, any ligand can be coated on fluorescent beads either directly or via antibodies. Binding between ligand-coated beads and cells can be measured by flow cytometry, which results in an easily quantifiable readout. Furthermore, it allows measuring of binding by specific cell subsets within a mixed cell population. Overall, this method is a convenient and easily standardized assay for measuring binding
AB - In this chapter we describe a fluorescent bead-binding assay, which is an efficient and feasible method to measure interaction between ligands and receptors on cells. In principle, any ligand can be coated on fluorescent beads either directly or via antibodies. Binding between ligand-coated beads and cells can be measured by flow cytometry, which results in an easily quantifiable readout. Furthermore, it allows measuring of binding by specific cell subsets within a mixed cell population. Overall, this method is a convenient and easily standardized assay for measuring binding
U2 - https://doi.org/10.1007/978-1-4939-3335-8_8
DO - https://doi.org/10.1007/978-1-4939-3335-8_8
M3 - Article
C2 - 26803626
SN - 1064-3745
VL - 1390
SP - 121
EP - 129
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -