Formation and clearance of TNF–TNF inhibitor complexes during TNF inhibitor treatment

Lea Catharina Berkhout, Merel Jeanne I'Ami, Simone Kruithof, Erik Hans Vogelzang, Femke Hooijberg, Margaretha Hendrika Louise Hart, Arthur Ebel Herman Bentlage, Debby Thomas, Severine Vermeire, Gestur Vidarsson, Anja ten Brinke, Michael Twahier Nurmohamed, Gerrit Jan Wolbink, Theo Rispens

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Abstract

Background and Purpose: Millions of patients with inflammatory diseases are treated with tumour necrosis factor (TNF) inhibitors (TNFi). Individual treatment response varies, in part related to variable drug clearance. The role of TNF–TNFi complexes in clearance of the different TNFi is controversial. Moreover, mechanistic insight into the structural aspects and biological significance of TNF–TNFi complexes is lacking. We hypothesized a role for Fc-mediated clearance of TNF–TNFi immune complexes. Therefore, we investigated circulating TNF–TNFi complexes upon treatment with certolizumab—lacking Fc tails—in comparison with adalimumab, golimumab, infliximab and etanercept. Experimental Approach: Drug-tolerant ELISAs were developed and used to quantify TNF during adalimumab, golimumab, etanercept, certolizumab and infliximab treatment in patients with inflammatory arthritis or ulcerative colitis for a maximum follow-up of 1 year. Effects on in vitro TNF production and Fc-mediated uptake of TNF–TNFi complexes were investigated for all five TNFi. Key Results: Circulating TNF concentrations were >20-fold higher during certolizumab treatment compared with adalimumab, reaching up to 23.1 ng·ml−1. Internalization of TNF–TNFi complexes by macrophages depended on Fc valency, with efficient uptake for the full antibody TNFi (three Fc tails), but little or no uptake for etanercept and certolizumab (one and zero Fc tail, respectively). TNF production was not affected by TNFi. Total TNF load did not affect clearance rate of total TNFi. Conclusions and Implications: Differences in TNFi structure profoundly affect clearance of TNF, while it is unlikely that TNF itself significantly contributes to target-mediated drug disposition of TNFi.
Original languageEnglish
JournalBritish journal of pharmacology
Early online date2023
DOIs
Publication statusE-pub ahead of print - 2023

Keywords

  • TNF inhibitor
  • anti-drug antibodies
  • clearance
  • immune complexes
  • pharmacokinetic
  • target-mediated drug disposition
  • tumour necrosis factor

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