TY - JOUR
T1 - Gene Therapy Strategies to Block HIV-1 Replication by RNA Interference
AU - Herrera-Carrillo, Elena
AU - Berkhout, Ben
PY - 2015
Y1 - 2015
N2 - The cellular mechanism of RNA interference (RNAi) plays an antiviral role in many organisms and can be used for the development of therapeutic strategies against viral pathogens. Persistent infections like the one caused by the human immunodeficiency virus type 1 (HIV-1) likely require a durable gene therapy approach. The continuous expression of the inhibitory RNA molecules in T cells is needed to effectively block HIV-1 replication. We discuss here several issues, ranging from the choice of RNAi inhibitor and vector system, finding the best target in the HIV-1 RNA genome, alternatively by targeting host mRNAs that encode important viral cofactors, to the setup of appropriate preclinical test systems. Finally, we briefly discuss the relevance of this topic for other viral pathogens that cause a chronic infection in humans
AB - The cellular mechanism of RNA interference (RNAi) plays an antiviral role in many organisms and can be used for the development of therapeutic strategies against viral pathogens. Persistent infections like the one caused by the human immunodeficiency virus type 1 (HIV-1) likely require a durable gene therapy approach. The continuous expression of the inhibitory RNA molecules in T cells is needed to effectively block HIV-1 replication. We discuss here several issues, ranging from the choice of RNAi inhibitor and vector system, finding the best target in the HIV-1 RNA genome, alternatively by targeting host mRNAs that encode important viral cofactors, to the setup of appropriate preclinical test systems. Finally, we briefly discuss the relevance of this topic for other viral pathogens that cause a chronic infection in humans
U2 - https://doi.org/10.1007/978-1-4939-2432-5_4
DO - https://doi.org/10.1007/978-1-4939-2432-5_4
M3 - Article
C2 - 25757616
SN - 0065-2598
VL - 848
SP - 71
EP - 95
JO - Advances in experimental medicine and biology
JF - Advances in experimental medicine and biology
ER -