Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

Marten Hansen; Eszter Varga; Tatjana Wüst; Nelleke Brouwer; Hugues Beauchemin; Clemens Mellink; Anne-Marie van der Kevie-Kersemaekers; Tarik Möröy; Bert van der Reijden; Marieke von Lindern; Emile van den Akker

doi:https://doi.org/10.1016/j.scr.2016.12.004

Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

Marten Hansen, Eszter Varga, Tatjana Wüst, Nelleke Brouwer, Hugues Beauchemin, Clemens Mellink, Anne-Marie van der Kevie-Kersemaekers, Tarik Möröy, Bert van der Reijden, Marieke von Lindern, Emile van den Akker

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts

Original language	English
Pages (from-to)	26-28
Journal	Stem Cell Research
Volume	18
DOIs	https://doi.org/10.1016/j.scr.2016.12.004
Publication status	Published - 2017

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https://doi.org/10.1016/j.scr.2016.12.004

Cite this

Hansen, M., Varga, E., Wüst, T., Brouwer, N., Beauchemin, H., Mellink, C., van der Kevie-Kersemaekers, A.-M., Möröy, T., van der Reijden, B., von Lindern, M., & van den Akker, E. (2017). Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts. Stem Cell Research, 18, 26-28. https://doi.org/10.1016/j.scr.2016.12.004

@article{266370f3437543f287a4fd594fbf440a,

title = "Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts",

abstract = "Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts",

author = "Marten Hansen and Eszter Varga and Tatjana W{\"u}st and Nelleke Brouwer and Hugues Beauchemin and Clemens Mellink and {van der Kevie-Kersemaekers}, Anne-Marie and Tarik M{\"o}r{\"o}y and {van der Reijden}, Bert and {von Lindern}, Marieke and {van den Akker}, Emile",

year = "2017",

doi = "https://doi.org/10.1016/j.scr.2016.12.004",

language = "English",

volume = "18",

pages = "26--28",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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Hansen, M, Varga, E, Wüst, T, Brouwer, N, Beauchemin, H, Mellink, C , van der Kevie-Kersemaekers, A-M, Möröy, T, van der Reijden, B, von Lindern, M & van den Akker, E 2017, 'Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts', Stem Cell Research, vol. 18, pp. 26-28. https://doi.org/10.1016/j.scr.2016.12.004

TY - JOUR

T1 - Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

AU - Hansen, Marten

AU - Varga, Eszter

AU - Wüst, Tatjana

AU - Brouwer, Nelleke

AU - Beauchemin, Hugues

AU - Mellink, Clemens

AU - van der Kevie-Kersemaekers, Anne-Marie

AU - Möröy, Tarik

AU - van der Reijden, Bert

AU - von Lindern, Marieke

AU - van den Akker, Emile

PY - 2017

Y1 - 2017

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DO - https://doi.org/10.1016/j.scr.2016.12.004

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