Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob

Taco G Uil, Toshiro Seki, Igor Dmitriev, Elena Kashentseva, Joanne T Douglas, Marianne G Rots, Jaap M Middeldorp, David T Curiel

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)


As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.

Original languageEnglish
Pages (from-to)121-4
Number of pages4
JournalCancer gene therapy
Issue number2
Publication statusPublished - Feb 2003


  • Adenoviridae
  • Antibodies, Monoclonal
  • Base Sequence
  • Capsid
  • Cytomegalovirus
  • DNA, Viral
  • Enzyme-Linked Immunosorbent Assay
  • Genetic Engineering
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Histidine
  • Journal Article
  • Ligands
  • Luciferases
  • Luminescent Proteins
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

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