Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob

Taco G Uil; Toshiro Seki; Igor Dmitriev; Elena Kashentseva; Joanne T Douglas; Marianne G Rots; Jaap M Middeldorp; David T Curiel

doi:https://doi.org/10.1038/sj.cgt.7700543

Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob

Taco G Uil, Toshiro Seki, Igor Dmitriev, Elena Kashentseva, Joanne T Douglas, Marianne G Rots, Jaap M Middeldorp, David T Curiel

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.

Original language	English
Pages (from-to)	121-4
Number of pages	4
Journal	Cancer gene therapy
Volume	10
Issue number	2
DOIs	https://doi.org/10.1038/sj.cgt.7700543
Publication status	Published - Feb 2003

Keywords

Adenoviridae
Antibodies, Monoclonal
Base Sequence
Capsid
Cytomegalovirus
DNA, Viral
Enzyme-Linked Immunosorbent Assay
Genetic Engineering
Genetic Vectors
Green Fluorescent Proteins
Histidine
Journal Article
Ligands
Luciferases
Luminescent Proteins
Molecular Sequence Data
Promoter Regions, Genetic
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Access to Document

https://doi.org/10.1038/sj.cgt.7700543

Cite this

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title = "Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob",

abstract = "As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.",

keywords = "Adenoviridae, Antibodies, Monoclonal, Base Sequence, Capsid, Cytomegalovirus, DNA, Viral, Enzyme-Linked Immunosorbent Assay, Genetic Engineering, Genetic Vectors, Green Fluorescent Proteins, Histidine, Journal Article, Ligands, Luciferases, Luminescent Proteins, Molecular Sequence Data, Promoter Regions, Genetic, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.",

author = "Uil, {Taco G} and Toshiro Seki and Igor Dmitriev and Elena Kashentseva and Douglas, {Joanne T} and Rots, {Marianne G} and Middeldorp, {Jaap M} and Curiel, {David T}",

year = "2003",

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doi = "https://doi.org/10.1038/sj.cgt.7700543",

language = "English",

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journal = "Cancer gene therapy",

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T1 - Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob

AU - Uil, Taco G

AU - Seki, Toshiro

AU - Dmitriev, Igor

AU - Kashentseva, Elena

AU - Douglas, Joanne T

AU - Rots, Marianne G

AU - Middeldorp, Jaap M

AU - Curiel, David T

PY - 2003/2

Y1 - 2003/2

N2 - As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.

AB - As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.

KW - Adenoviridae

KW - Antibodies, Monoclonal

KW - Base Sequence

KW - Capsid

KW - Cytomegalovirus

KW - DNA, Viral

KW - Enzyme-Linked Immunosorbent Assay

KW - Genetic Engineering

KW - Genetic Vectors

KW - Green Fluorescent Proteins

KW - Histidine

KW - Journal Article

KW - Ligands

KW - Luciferases

KW - Luminescent Proteins

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

U2 - https://doi.org/10.1038/sj.cgt.7700543

DO - https://doi.org/10.1038/sj.cgt.7700543

M3 - Article

C2 - 12536200

SN - 0929-1903

VL - 10

SP - 121

EP - 124

JO - Cancer gene therapy

JF - Cancer gene therapy

IS - 2

ER -