TY - JOUR
T1 - Generation of human erythroblast-derived iPSC line using episomal reprogramming system
AU - Varga, Eszter
AU - Hansen, Marten
AU - Wüst, Tatjana
AU - von Lindern, Marieke
AU - van den Akker, Emile
PY - 2017
Y1 - 2017
N2 - Peripheral bloodmononuclear cellswere isolated and cultured to a pure pro-EBL population and reprogrammed using episomal plasmids. The pluripotency of transgene-free induced pluripotent stem cell (iPSC) line was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. The iPSC line showed normal karyotype. Peripheral blood is a non-invasive easy accessible cell source and combined with EBL outgrowth in vitro, a routine process obtaining sufficient amount of homogenous cells can be obtained within a week. Using episomal delivery, pro-EBLs can be reprogrammed in a transgene-free, cost effective system. (c) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
AB - Peripheral bloodmononuclear cellswere isolated and cultured to a pure pro-EBL population and reprogrammed using episomal plasmids. The pluripotency of transgene-free induced pluripotent stem cell (iPSC) line was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. The iPSC line showed normal karyotype. Peripheral blood is a non-invasive easy accessible cell source and combined with EBL outgrowth in vitro, a routine process obtaining sufficient amount of homogenous cells can be obtained within a week. Using episomal delivery, pro-EBLs can be reprogrammed in a transgene-free, cost effective system. (c) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
U2 - https://doi.org/10.1016/j.scr.2017.10.001
DO - https://doi.org/10.1016/j.scr.2017.10.001
M3 - Article
C2 - 29040913
SN - 1873-5061
VL - 25
SP - 30
EP - 33
JO - Stem Cell Research
JF - Stem Cell Research
ER -