TY - JOUR
T1 - Genomic and microenvironmental landscape of stage I follicular lymphoma, compared with stage III/IV
AU - Los-de Vries, G. Tjitske
AU - Stevens, Wendy B. C.
AU - van Dijk, Erik
AU - Langois-Jacques, Carole
AU - Clear, Andrew J.
AU - Stathi, Phylicia
AU - Roemer, Margaretha G. M.
AU - Mendeville, Matias
AU - Hijmering, Nathalie J.
AU - Sander, Birgitta
AU - Rosenwald, Andreas
AU - Calaminici, Maria
AU - Hoster, Eva
AU - Hiddemann, Wolfgang
AU - Gaulard, Philippe
AU - Salles, Gilles
AU - Horn, Heike
AU - Klapper, Wolfram
AU - Xerri, Luc
AU - Burton, Catherine
AU - Tooze, Reuben M.
AU - Smith, Alexandra G.
AU - Buske, Christian
AU - Scott, David W.
AU - Natkunam, Yasodha
AU - Advani, Ranjana
AU - Sehn, Laurie H.
AU - Raemaekers, John
AU - Gribben, John
AU - Kimby, Eva
AU - Kersten, Marie José
AU - Maucort-Boulch, Delphine
AU - Ylstra, Bauke
AU - de Jong, Daphne
N1 - Funding Information: This study was supported by the Dutch Cancer Society (grant KWF 2015-7925) and by unrestricted grants from van Vlissingen Lymfoom Fonds, Genentech/Roche, GlaxoSmithKline, Pfizer Pharma, Teva Pharmaceuticals/Cephalon, Millenium Pharmaceuticals Inc., and Celgene. The HMRN is funded by Cancer Research UK (grant 29685) and Blood Cancer UK (grant 1503). Funding Information: The LLBC is a collaboration of 9 international lymphoma research groups, each represented by a clinical investigator and one or more hematopathologists and supported by a team of statisticians. Foundation of the LLBC was made possible with a grant from the Van Vlissingen Lymphoma Foundation. EORTC Lymphoma group represented by: Daphne de Jong and John Raemaekers. HOVON Lymphoma group represented by: Daphne de Jong and Marie-José Kersten. Lymphoma Study Association represented by: Philippe Gaulard, Gilles Salles, Luc Xerri, Delphine Maucort-Boulch, and Carole Langois-Jacques. British Columbia Cancer Agency represented by: Laurie H. Sehn and David W. Scott. GLSG represented by: Andreas Rosenwald, Wolfram Klapper, Christian Buske, Wolfgang Hiddemann, Eva Hoster, and Heike Horn. Nordic lymphoma group represented by: Birgitta Sander and Eva Kimby. Barts Cancer Institute represented by: Maria Calaminici, John Gribben, and Andrew J. Clear. HMRN represented by: Catherine Burton, Reuben M. Tooze, Funding Information: G.T.L.-d.V., D.d.J., B.Y., and M.J.K. received funding from the Dutch Cancer Society (grant KWF 2015-7925). W.B.C.S., E.v.D., C.L.-J., and D.M.-J. were funded by unrestricted grants from Vlissingen Lymfoom Fonds, Genentech/Roche, GlaxoS-mithKline, Pfizer Pharma, Teva Pharmaceuticals/Cephalon, Millenium Pharmaceuticals Inc., and Celgene. A.G.S. was funded by Malignancy Research Network is funded by Cancer Research UK, grant numbers 29685; and Blood Cancer UK, grant number 1503. Publisher Copyright: © 2022 American Society of Hematology. All rights reserved.
PY - 2022/9/27
Y1 - 2022/9/27
N2 - Although the genomic and immunemicroenvironmental landscape of follicular lymphoma (FL) has been extensively investigated, little is known about the potential biological differences between stage I and stage III/IV disease. Using next-generation sequencing and immunohistochemistry, 82 FL nodal stage I caseswere analyzed and comparedwith 139 FL stage III/IV nodal cases.Many similarities in mutations, chromosomal copy number aberrations, and microenvironmental cell populationswere detected. However, therewere also significant differences inmicroenvironmental and genomic features. CD8- T cells (P= .02) and STAT6 mutations (false discovery rate [FDR],0.001)weremore frequent in stage I FL. In contrast, programmed cell death protein 1-positive T cells, CD68-/CD163- macrophages (P<.001), BCL2 translocation (BCL2trl-) (P< .0001), and KMT2D (FDR= 0.003) and CREBBP (FDR= 0.04) mutationswere foundmore frequently in stage III/IV FL. Using clustering,we identified 3 clusters within stage I, and 2 clusterswithin stage III/IV. The BLC2trl- stage I clusterwas comparable to the BCL2trl- cluster in stage III/IV. The two BCL2trl- stage I clusters were unique for stage I. One was enriched for CREBBP (95%) andSTAT6 (64%)mutations,without BLC6 translocation (BCL6trl), whereas the BCL2trl- stage III/IV cluster contained BCL6trl (64%)with fewer CREBBP (45%) andSTAT6 (9%)mutations. The other BCL2trl- stage I clusterwas relatively heterogeneouswith more copy number aberrations and linker histonemutations. This exploratory study shows that stage I FL is genetically heterogeneouswith different underlying oncogenic pathways. Stage I FL BCL2trl- is likely STAT6 driven,whereas BCL2trl- stage III/IV appears to bemore BCL6trl driven.
AB - Although the genomic and immunemicroenvironmental landscape of follicular lymphoma (FL) has been extensively investigated, little is known about the potential biological differences between stage I and stage III/IV disease. Using next-generation sequencing and immunohistochemistry, 82 FL nodal stage I caseswere analyzed and comparedwith 139 FL stage III/IV nodal cases.Many similarities in mutations, chromosomal copy number aberrations, and microenvironmental cell populationswere detected. However, therewere also significant differences inmicroenvironmental and genomic features. CD8- T cells (P= .02) and STAT6 mutations (false discovery rate [FDR],0.001)weremore frequent in stage I FL. In contrast, programmed cell death protein 1-positive T cells, CD68-/CD163- macrophages (P<.001), BCL2 translocation (BCL2trl-) (P< .0001), and KMT2D (FDR= 0.003) and CREBBP (FDR= 0.04) mutationswere foundmore frequently in stage III/IV FL. Using clustering,we identified 3 clusters within stage I, and 2 clusterswithin stage III/IV. The BLC2trl- stage I clusterwas comparable to the BCL2trl- cluster in stage III/IV. The two BCL2trl- stage I clusters were unique for stage I. One was enriched for CREBBP (95%) andSTAT6 (64%)mutations,without BLC6 translocation (BCL6trl), whereas the BCL2trl- stage III/IV cluster contained BCL6trl (64%)with fewer CREBBP (45%) andSTAT6 (9%)mutations. The other BCL2trl- stage I clusterwas relatively heterogeneouswith more copy number aberrations and linker histonemutations. This exploratory study shows that stage I FL is genetically heterogeneouswith different underlying oncogenic pathways. Stage I FL BCL2trl- is likely STAT6 driven,whereas BCL2trl- stage III/IV appears to bemore BCL6trl driven.
UR - http://www.scopus.com/inward/record.url?scp=85139389244&partnerID=8YFLogxK
U2 - https://doi.org/10.1182/bloodadvances.2022008355
DO - https://doi.org/10.1182/bloodadvances.2022008355
M3 - Article
C2 - 35816682
SN - 2473-9529
VL - 6
SP - 5482
EP - 5493
JO - Blood advances
JF - Blood advances
IS - 18
ER -