@article{c12ce7a55e344eadbcf29069f2bcf288,
title = "GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function",
abstract = "The Ig superfamily protein glycoprotein A33 (GPA33) has been implicated in immune dysregulation, but little is known about its expression in the immune compartment. Here, we comprehensively determined GPA33 expression patterns on human blood leukocyte subsets, using mass and flow cytometry. We found that GPA33 was expressed on fractions of B, dendritic, natural killer and innate lymphoid cells. Most prominent expression was found in the CD4+ T cell compartment. Na{\"i}ve and CXCR5+ regulatory T cells were GPA33high, and na{\"i}ve conventional CD4+ T cells expressed intermediate GPA33 levels. The expression pattern of GPA33 identified functional heterogeneity within the CD4+ central memory T cell (Tcm) population. GPA33+ CD4+ Tcm cells were fully undifferentiated, bona fide Tcm cells that lack immediate effector function, whereas GPA33– Tcm cells exhibited rapid effector functions and may represent an early stage of differentiation into effector/effector memory T cells before loss of CD62L. Expression of GPA33 in conventional CD4+ T cells suggests a role in localization and/or preservation of an undifferentiated state. These results form a basis to study the function of GPA33 and show it to be a useful marker to discriminate between different cellular subsets, especially in the CD4+ T cell lineage.",
keywords = "CD4 T cell differentiation, CyTOF, antibody therapy, flow cytometry, human glycoprotein A33",
author = "Rianne Opstelten and Suwandi, {Jessica S.} and Slot, {Manon C.} and Florencia Morgana and Scott, {Andrew M.} and Sandra Laban and Tatjana Nikolic and Turksma, {Annelies W.} and Anna Kroeze and Carlijn Voermans and Jaap-Jan Zwaginga and Roep, {Bart O.} and Derk Amsen",
note = "Funding Information: We would like to thank Mette Hazenberg and Vera van Hoeven for their expert opinion on the ILC data, Martijn Nolte for advice, and Anja ten Brinke and Naomi Weterings for helping to set up and validate the FACS panels. This work was partly supported by grants from the Landsteiner Foundation for Blood Cell Research to DA (LSBR 1430, LSBR 1818). Furthermore, J.S. and B.R. are financed by the Dutch Diabetes Research Foundation and Foundation DON (Expert Center Grant, 2013.40.1693), T.N. and J.J.Z. are financed by the Dutch Arthritis Foundation (Research Center of Excellence program, LLP-16). A.K. was supported by Sanquin Research (PPOC13-027 grant). A Fellowship from the Landsteiner Foundation for Blood Transfusion Research to CV (grant no. #1101) also supported part of this work. A.M.S. is supported by an NHMRC Investigator Grant (No: 1177837). The graphical abstract was created with the BioRender software. Funding Information: We would like to thank Mette Hazenberg and Vera van Hoeven for their expert opinion on the ILC data, Martijn Nolte for advice, and Anja ten Brinke and Naomi Weterings for helping to set up and validate the FACS panels. This work was partly supported by grants from the Landsteiner Foundation for Blood Cell Research to DA (LSBR 1430, LSBR 1818). Furthermore, J.S. and B.R. are financed by the Dutch Diabetes Research Foundation and Foundation DON (Expert Center Grant, 2013.40.1693), T.N. and J.J.Z. are financed by the Dutch Arthritis Foundation (Research Center of Excellence program, LLP‐16). A.K. was supported by Sanquin Research (PPOC13‐027 grant). A Fellowship from the Landsteiner Foundation for Blood Transfusion Research to CV (grant no. #1101) also supported part of this work. A.M.S. is supported by an NHMRC Investigator Grant (No: 1177837). The graphical abstract was created with the BioRender software. Publisher Copyright: {\textcopyright} 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = jun,
doi = "https://doi.org/10.1002/eji.202048744",
language = "English",
volume = "51",
pages = "1377--1389",
journal = "European journal of immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "6",
}