H9c2 cardiomyoblasts produce thyroid hormone

Christof Meischl, Henk P. Buermans, Thierry Hazes, Marian J. Zuidwijk, René J. P. Musters, Christa Boer, Arthur van Lingen, Warner S. Simonides, Marinus A. Blankenstein, Corrine Dupuy, Walter J. Paulus, C. Erik Hack, Carrie Ris-Stalpers, Dirk Roos, Hans W. M. Niessen

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)


Thyroid hormone acts on a wide range of tissues. In the cardiovascular system, thyroid hormone is an important regulator of cardiac function and cardiovascular hemodynamics. Although some early reports in the literature suggested an unknown extrathyroidal source of thyroid hormone, it is currently thought to be produced exclusively in the thyroid gland, a highly specialized organ with the sole function of generating, storing, and secreting thyroid hormone. Whereas most of the proteins necessary for thyroid hormone synthesis are thought to be expressed exclusively in the thyroid gland, we now have found evidence that all of these proteins, i.e., thyroglobulin, DUOX1, DUOX2, the sodium-iodide symporter, pendrin, thyroid peroxidase, and thyroid-stimulating hormone receptor, are also expressed in cardiomyocytes. Furthermore, we found thyroglobulin to be transiently upregulated in an in vitro model of ischemia. When performing these experiments in the presence of 125 I, we found that 125 I was integrated into thyroglobulin and that under ischemia-like conditions the radioactive signal in thyroglobulin was reduced. Concomitantly we observed an increase of intracellularly produced, 125 I-labeled thyroid hormone. In conclusion, our findings demonstrate for the first time that cardiomyocytes produce thyroid hormone in a manner adapted to the cell's environment
Original languageEnglish
Pages (from-to)C1227-C1233
JournalAmerican journal of physiology. Cell physiology
Issue number5
Publication statusPublished - May 2008


  • Animals
  • Cell Culture Techniques
  • DNA, Complementary
  • Dual Oxidases
  • Flavoproteins
  • Iodine Radioisotopes
  • Journal Article
  • Male
  • Myocytes, Cardiac
  • NADPH Oxidases
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Rats
  • Rats, Wistar
  • Research Support, Non-U.S. Gov't
  • Thyroglobulin
  • Thyroid Hormones

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