TY - JOUR
T1 - Hematopoietic stem cell expansion: Challenges and opportunities
AU - Walasek, Marta A.
AU - van Os, Ronald
AU - de Haan, Gerald
PY - 2012
Y1 - 2012
N2 - Attempts to improve hematopoietic reconstitution and engraftment potential of ex vivo-expanded hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful due to the inability to generate sufficient stem cell numbers and to excessive differentiation of the starting cell population. Although hematopoietic stem cells (HSCs) will rapidly expand after in vivo transplantation, experience from in vitro studies indicates that control of HSPC self-renewal and differentiation in culture remains difficult. Protocols that are based on hematopoietic cytokines have failed to support reliable amplification of immature stem cells in culture, suggesting that additional factors are required. In recent years, several novel factors, including developmental factors and chemical compounds, have been reported to affect HSC self-renewal and improve ex vivo stem cell expansion protocols. Here, we highlight early expansion attempts and review recent development in the extrinsic control of HSPC fate in vitro. © 2012 New York Academy of Sciences.
AB - Attempts to improve hematopoietic reconstitution and engraftment potential of ex vivo-expanded hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful due to the inability to generate sufficient stem cell numbers and to excessive differentiation of the starting cell population. Although hematopoietic stem cells (HSCs) will rapidly expand after in vivo transplantation, experience from in vitro studies indicates that control of HSPC self-renewal and differentiation in culture remains difficult. Protocols that are based on hematopoietic cytokines have failed to support reliable amplification of immature stem cells in culture, suggesting that additional factors are required. In recent years, several novel factors, including developmental factors and chemical compounds, have been reported to affect HSC self-renewal and improve ex vivo stem cell expansion protocols. Here, we highlight early expansion attempts and review recent development in the extrinsic control of HSPC fate in vitro. © 2012 New York Academy of Sciences.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865370552&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/22901265
U2 - https://doi.org/10.1111/j.1749-6632.2012.06549.x
DO - https://doi.org/10.1111/j.1749-6632.2012.06549.x
M3 - Article
C2 - 22901265
SN - 0077-8923
VL - 1266
SP - 138
EP - 150
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
IS - 1
ER -