High levels of osteoprotegerin are associated with coronary artery calcification in patients suspected of a chronic coronary syndrome

M. Dekker, F. Waissi, M.J.M. Silvis, J.V. Bennekom, A.H. Schoneveld, R.J. de Winter, I. Isgum, N. Lessmann, B.K. Velthuis, G. Pasterkamp, A. Mosterd, L. Timmers, D.P.V. de Kleijn

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Plasma osteoprotegerin (OPG) and vascular smooth muscle cell (VSMC) derived extracellular vesicles (EVs) are important regulators in the process of vascular calcification (VC). In population studies, high levels of OPG are associated with events. In animal studies, however, high OPG levels result in reduction of VC. VSMC-derived EVs are assumed to be responsible for OPG transport and VC but this role has not been studied. For this, we investigated the association between OPG in plasma and circulating EVs with coronary artery calcium (CAC) as surrogate for VC in symptomatic patients. We retrospectively assessed 742 patients undergoing myocardial perfusion imaging (MPI). CAC scores were determined on the MPI-CT images using a previously developed automated algorithm. Levels of OPG were quantified in plasma and two EV-subpopulations (LDL and TEX), using an electrochemiluminescence immunoassay. Circulating levels of OPG were independently associated with CAC scores in plasma; OR 1.39 (95% CI 1.17-1.65), and both EV populations; EV-LDL; OR 1.51 (95% CI 1.27-1.80) and EV-TEX; OR 1.21 (95% CI 1.02-1.42). High levels of OPG in plasma were independently associated with CAC scores in this symptomatic patient cohort. High levels of EV-derived OPG showed the same positive association with CAC scores, suggesting that EV-derived OPG mirrors the same pathophysiological process as plasma OPG.

Original languageEnglish
Article number18946
Number of pages10
JournalScientific reports
Volume11
Issue number1
DOIs
Publication statusPublished - 23 Sept 2021

Keywords

  • Aged
  • Biomarkers/blood
  • Chronic Disease
  • Coronary Artery Disease/blood
  • Coronary Vessels/diagnostic imaging
  • Extracellular Vesicles/metabolism
  • Female
  • Humans
  • Male
  • Middle Aged
  • Myocardial Perfusion Imaging
  • Osteoprotegerin/blood
  • Prospective Studies
  • Retrospective Studies
  • Risk Assessment/methods
  • Risk Factors
  • Syndrome
  • Vascular Calcification/blood

Cite this