TY - JOUR
T1 - High-quality human preimplantation embryos actively influence endometrial stromal cell migration
AU - Berkhout, R. P.
AU - Lambalk, C. B.
AU - Huirne, J.
AU - Mijatovic, V.
AU - Repping, S.
AU - Hamer, G.
AU - Mastenbroek, S.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Purpose: The purpose of this paper is to study whether human preimplantation embryos regulate endometrial stromal cell (hESC) migration. Methods: Primary hESCs were isolated from fertile patients undergoing hysterectomy for benign conditions (uterine scar niche n = 3, dysmenorrhea n = 2; no hormonal treatment). Migration and proliferation assays were performed by culturing decidualized or non-decidualized hESCs in the presence of embryo conditioned medium (ECM) from high-quality embryos (fragmentation ≤ 20%) or from low-quality embryos (fragmentation > 20%) or in non-conditioned medium from the same dishes (control). ECM samples from 425 individually cultured human embryos were used in this study. Results: ECM from high-quality embryos, i.e., with a low percentage of fragmentation, actively stimulated decidualized hESC migration (p < 0.001). This effect was consistent throughout embryonic development from cleavage stage embryos with 2–7 cells (high quality vs. control; p = 0.036), 8–18 cells (high quality vs. control; p < 0.001) to morulae (high quality vs. control; p = 0.003). Additionally, linear regression analysis showed that hESC migration was influenced by embryo quality (fragmentation, β − 0.299; p = 0.025) and not developmental stage (cell number, β 0.177; p = 0.176) or maternal age (β − 0.036; p = 0.78). Opposite to decidualized hESCs, the migration response of non-decidualized hESCs was inhibited by ECM from high-quality embryos (p = 0.019). ECM from low-quality embryos, i.e., with a high percentage of fragmentation, did not cause an altered migration response in decidualized hESCs (p = 0.860) or non-decidualized hESCs (p = 0.986). Furthermore, ECM of both high- and low-quality human embryos did not influence the number of proliferating cells (p = 0.375) and the cell cycle time (p = 0.297) of non-decidualized or decidualized hESCs. Conclusion: This study reveals a mechanism by which high-quality human preimplantation embryos actively interact with the endometrium to increase their chances of successful implantation.
AB - Purpose: The purpose of this paper is to study whether human preimplantation embryos regulate endometrial stromal cell (hESC) migration. Methods: Primary hESCs were isolated from fertile patients undergoing hysterectomy for benign conditions (uterine scar niche n = 3, dysmenorrhea n = 2; no hormonal treatment). Migration and proliferation assays were performed by culturing decidualized or non-decidualized hESCs in the presence of embryo conditioned medium (ECM) from high-quality embryos (fragmentation ≤ 20%) or from low-quality embryos (fragmentation > 20%) or in non-conditioned medium from the same dishes (control). ECM samples from 425 individually cultured human embryos were used in this study. Results: ECM from high-quality embryos, i.e., with a low percentage of fragmentation, actively stimulated decidualized hESC migration (p < 0.001). This effect was consistent throughout embryonic development from cleavage stage embryos with 2–7 cells (high quality vs. control; p = 0.036), 8–18 cells (high quality vs. control; p < 0.001) to morulae (high quality vs. control; p = 0.003). Additionally, linear regression analysis showed that hESC migration was influenced by embryo quality (fragmentation, β − 0.299; p = 0.025) and not developmental stage (cell number, β 0.177; p = 0.176) or maternal age (β − 0.036; p = 0.78). Opposite to decidualized hESCs, the migration response of non-decidualized hESCs was inhibited by ECM from high-quality embryos (p = 0.019). ECM from low-quality embryos, i.e., with a high percentage of fragmentation, did not cause an altered migration response in decidualized hESCs (p = 0.860) or non-decidualized hESCs (p = 0.986). Furthermore, ECM of both high- and low-quality human embryos did not influence the number of proliferating cells (p = 0.375) and the cell cycle time (p = 0.297) of non-decidualized or decidualized hESCs. Conclusion: This study reveals a mechanism by which high-quality human preimplantation embryos actively interact with the endometrium to increase their chances of successful implantation.
KW - Cross-talk
KW - Decidualization
KW - Embryo
KW - Endometrium
KW - Implantation
UR - http://www.scopus.com/inward/record.url?scp=85039843636&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/s10815-017-1107-z
DO - https://doi.org/10.1007/s10815-017-1107-z
M3 - Article
C2 - 29282583
SN - 1058-0468
VL - 35
SP - 659
EP - 667
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 4
ER -