High-Throughput Firefly Luciferase Reporter Assays

Ellen Siebring-van Olst; Victor W van Beusechem

doi:https://doi.org/10.1007/978-1-4939-7724-6_2

High-Throughput Firefly Luciferase Reporter Assays

Ellen Siebring-van Olst, Victor W van Beusechem

Research output: Contribution to journal › Article › Academic

3 Citations (Scopus)

Abstract

Firefly luciferase reporter gene assays find wide application in high-throughput screens to identify molecular components of biological networks or to identify chemical compounds capable of interfering with cellular signaling. Here, we present methods to prepare affordable firefly luciferase assay reagents and procedures to use these reagents in reporter gene high-throughput screening with large batches of 96-well cell culture plates.

Original language	English
Pages (from-to)	19-29
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1755
DOIs	https://doi.org/10.1007/978-1-4939-7724-6_2
Publication status	Published - 2018

Access to Document

https://doi.org/10.1007/978-1-4939-7724-6_2

Cite this

@article{b0ecb469925c453f9704dc104eda6868,

title = "High-Throughput Firefly Luciferase Reporter Assays",

abstract = "Firefly luciferase reporter gene assays find wide application in high-throughput screens to identify molecular components of biological networks or to identify chemical compounds capable of interfering with cellular signaling. Here, we present methods to prepare affordable firefly luciferase assay reagents and procedures to use these reagents in reporter gene high-throughput screening with large batches of 96-well cell culture plates.",

author = "{Siebring-van Olst}, Ellen and {van Beusechem}, {Victor W}",

year = "2018",

doi = "https://doi.org/10.1007/978-1-4939-7724-6_2",

language = "English",

volume = "1755",

pages = "19--29",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - High-Throughput Firefly Luciferase Reporter Assays

AU - Siebring-van Olst, Ellen

AU - van Beusechem, Victor W

PY - 2018

Y1 - 2018

N2 - Firefly luciferase reporter gene assays find wide application in high-throughput screens to identify molecular components of biological networks or to identify chemical compounds capable of interfering with cellular signaling. Here, we present methods to prepare affordable firefly luciferase assay reagents and procedures to use these reagents in reporter gene high-throughput screening with large batches of 96-well cell culture plates.

AB - Firefly luciferase reporter gene assays find wide application in high-throughput screens to identify molecular components of biological networks or to identify chemical compounds capable of interfering with cellular signaling. Here, we present methods to prepare affordable firefly luciferase assay reagents and procedures to use these reagents in reporter gene high-throughput screening with large batches of 96-well cell culture plates.

U2 - https://doi.org/10.1007/978-1-4939-7724-6_2

DO - https://doi.org/10.1007/978-1-4939-7724-6_2

M3 - Article

C2 - 29671260

SN - 1064-3745

VL - 1755

SP - 19

EP - 29

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -