Host shutoff during productive Epstein-Barr virus infection is mediated by BGLF5 and may contribute to immune evasion

Martin Rowe, Britt Glaunsinger, Daphne van Leeuwen, Jianmin Zuo, David Sweetman, Don Ganem, Jaap Middeldorp, Emmanuel J H J Wiertz, Maaike E Ressing

Research output: Contribution to journalArticleAcademicpeer-review

189 Citations (Scopus)

Abstract

Relatively little is known about immune evasion during the productive phase of infection by the gamma(1)-herpesvirus Epstein-Barr virus (EBV). The use of a unique system to isolate cells in lytic cycle allowed us to identify a host shutoff function operating in productively EBV-infected B cells. This impairment of protein synthesis results from mRNA degradation induced upon expression of the early lytic-cycle gene product BGLF5. Recently, a gamma(2)-herpesvirus, Kaposi sarcoma herpesvirus, has also been shown to encode a host shutoff function, indicating that host shutoff appears to be a general feature of gamma-herpesviruses. One of the consequences of host shutoff is a block in the synthesis of HLA class I and II molecules, reflected by reduced levels of these antigen-presenting complexes at the surface of cells in EBV lytic cycle. This effect could lead to escape from T cell recognition and elimination of EBV-producing cells, thereby allowing generation of viral progeny in the face of memory T cell responses.

Original languageEnglish
Pages (from-to)3366-71
Number of pages6
JournalPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume104
Issue number9
DOIs
Publication statusPublished - 27 Feb 2007

Keywords

  • Antigen Presentation
  • B-Lymphocytes
  • Cell Line, Tumor
  • Comparative Study
  • Deoxyribonucleases
  • Epstein-Barr Virus Infections
  • Gene Expression Regulation
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Humans
  • Journal Article
  • Microscopy, Fluorescence
  • Protein Biosynthesis
  • Research Support, Non-U.S. Gov't
  • Viral Proteins

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