TY - JOUR
T1 - How to Reliably Define Human CD8+ T-Cell Subsets
T2 - Markers Playing Tricks
AU - van Aalderen, Michiel C.
AU - van Lier, Rene A. W.
AU - Hombrink, Pleun
N1 - Publisher Copyright: Copyright © 2021 Cold Spring Harbor Laboratory Press; all rights reserved.
PY - 2021/11/1
Y1 - 2021/11/1
N2 - In recent years, our understanding about the functional complexity of CD8+ T-cell populations has increased tremendously. The immunology field is now facing challenges to translate these insights into phenotypic definitions that correlate reliably with distinct functional traits. This is key to adequately monitor and understand compound immune responses including vaccination and immunotherapy regimens. Here we will summarize our understanding of the current state in the human CD8+ T-cell subset characterization field. We will address how reliably the currently used cell surface markers are connected to differentiation status and function of particular subsets. By restricting ourselves to CD8+ αβ T cells, we will focus mostly on major histocompatibility complex (MHC) class I-restricted virus- and tumor-specific T cells. This comes with a major advantage as fluorescently labeled peptide-loaded MHC class I multimers have been widely used to identify and characterize these cells.
AB - In recent years, our understanding about the functional complexity of CD8+ T-cell populations has increased tremendously. The immunology field is now facing challenges to translate these insights into phenotypic definitions that correlate reliably with distinct functional traits. This is key to adequately monitor and understand compound immune responses including vaccination and immunotherapy regimens. Here we will summarize our understanding of the current state in the human CD8+ T-cell subset characterization field. We will address how reliably the currently used cell surface markers are connected to differentiation status and function of particular subsets. By restricting ourselves to CD8+ αβ T cells, we will focus mostly on major histocompatibility complex (MHC) class I-restricted virus- and tumor-specific T cells. This comes with a major advantage as fluorescently labeled peptide-loaded MHC class I multimers have been widely used to identify and characterize these cells.
UR - http://www.scopus.com/inward/record.url?scp=85121407133&partnerID=8YFLogxK
U2 - https://doi.org/10.1101/cshperspect.a037747
DO - https://doi.org/10.1101/cshperspect.a037747
M3 - Review article
C2 - 33782028
SN - 1943-0264
VL - 13
JO - Cold Spring Harbor Perspectives in Biology
JF - Cold Spring Harbor Perspectives in Biology
IS - 11
ER -