Human CD8(+) T cell responses against five newly identified respiratory syncytial virus-derived epitopes

Jojanneke Heidema, Godelieve J. de Bree, Patricia M. A. de Graaff, Wendy W. C. van Maren, Peter Hoogerhout, Theo A. Out, Jan L. L. Kimpen, Grada M. van Bleek

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Abstract

CD8(+) T lymphocytes play a major role in the clearance of respiratory syncytial virus (RSV) infections. To be able to study the primary CTL response in RSV-infected children, epitopes presented by a set of commonly used HLA alleles (HLA-A1, -A3, -B44 and -B51) were searched for. Five epitopes were characterized derived from the matrix (M), non-structural (NS2) and second matrix (M2) proteins of RSV. All epitopes were shown to be processed and presented by RSV-infected antigen-presenting cells. HLA-A1 tetramers for one of these epitopes derived from the M protein were constructed and used to quantify and phenotype the memory CD8(+) T cell pool in a panel of healthy adult donors. In about 60 % of the donors, CD8(+) T cells specific for the M protein could be identified. These cells belonged to the memory T cell subset characterized by expression of CD27 and CD28, and down-regulation of CCR7 and CD45RA. The frequency of tetramer-positive cells varied between 0.4 and 3 per 10(4) CD8(+) T cells in PBMC of healthy asymptomatic adult donors
Original languageEnglish
Pages (from-to)2365-2374
JournalJournal of general virology
Volume85
Issue numberPart 8
DOIs
Publication statusPublished - 2004

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