TY - JOUR
T1 - Human immunodeficiency virus-negative men who have sex with men have an altered T-Cell phenotype and bioenergy metabolism
AU - Kruize, Zita
AU - Maurer, Irma
AU - van Dort, Karel A.
AU - van den Elshout, Mark A. M.
AU - Hoornenborg, Elske
AU - Booiman, Thijs
AU - Prins, Maria
AU - Kootstra, Neeltje A.
N1 - Funding Information: Financial support. This work was funded by Aids Fonds (Grant Number P-22301). Funding Information: We thank all participants of the Amsterdam Cohort studies for their contribution. The Amsterdam Cohort Studies on HIV infection and AIDS, a collaboration between the Public Health Service Amsterdam, the Amsterdam UMC of the University of Amsterdam, Sanquin Blood Supply Foundation, Medical Center Jan van Goyen, and the HIV Focus Center of the DC-Clinics, are part of the Netherlands HIV Monitoring Foundation and financially supported by the Center for Infectious Disease Control of the Netherlands National Institute for Public Health and the Environment. Publisher Copyright: © The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Background. We recently reported that the levels of activation, exhaustion, and terminal differentiation within the peripheral T-cell compartment were increased in men who have sex with men (MSM) compared with blood bank donors. During activation and differentiation, T cells undergo metabolic changes to maintain their energy demand. Methods. The effect of cytomeglovirus (CMV) infection and risk behavior on the immune phenotype of peripheral T cells and the immune bioenergy metabolism profile in human immunodeficiency virus-negative MSM (with high or low sexual risk behavior) and blood bank donors was evaluated. Results. Men who have sex with men exhibited increased levels of T-cell activation and terminal differentiation and an impairment of the bioenergy metabolism (mitochondrial respiration and glycolysis) compared with blood bank donors. Cytomeglovirus infection was associated with increased terminal differentiation of CD4 + (B = 3.41; 95% confidence interval [CI], 1.98-4.85; P <.0001) and CD8 + T cells (CD57 +: B = 1.21, 95% CI = 0.41-2.02, P =.004; CD27 −CD28 −: B = 2.20, 95% CI = 1.21-3.18, P <.0001; and CD57 + of CD28 −: B = 1.02, 95% CI = 0.38-1.66, P =.002) and increased glycolysis (B = 0.97; 95% CI, 0.27-1.67; P =.007). Risk behavior was associated with increase activation of CD4 + T cells (B = 0.22; 95% CI, 0.07-0.37; P =.005), increased terminal differentiation of CD4 + (B = 0.82; 95% CI, 0.44-1.20; P <.0001) and CD8 + T cells (B = 1.55; 95% CI, 0.58-2.51; P =.002), and decreased glycolysis (glycolysis: B = −0.40, 95% CI = −0.68 to 0.12, P =.006; and glycolytic capacity: B = −0.54, 95% CI = −0.91 to 0.16, P =.005). Conclusions. Men who have sex with men show an increased prevalence of bloodborne and sexually transmitted infection, indicating that immunological changes in the T-cell population and the bioenergy metabolism observed in MSM can most likely be attributed to chronic antigen exposure.
AB - Background. We recently reported that the levels of activation, exhaustion, and terminal differentiation within the peripheral T-cell compartment were increased in men who have sex with men (MSM) compared with blood bank donors. During activation and differentiation, T cells undergo metabolic changes to maintain their energy demand. Methods. The effect of cytomeglovirus (CMV) infection and risk behavior on the immune phenotype of peripheral T cells and the immune bioenergy metabolism profile in human immunodeficiency virus-negative MSM (with high or low sexual risk behavior) and blood bank donors was evaluated. Results. Men who have sex with men exhibited increased levels of T-cell activation and terminal differentiation and an impairment of the bioenergy metabolism (mitochondrial respiration and glycolysis) compared with blood bank donors. Cytomeglovirus infection was associated with increased terminal differentiation of CD4 + (B = 3.41; 95% confidence interval [CI], 1.98-4.85; P <.0001) and CD8 + T cells (CD57 +: B = 1.21, 95% CI = 0.41-2.02, P =.004; CD27 −CD28 −: B = 2.20, 95% CI = 1.21-3.18, P <.0001; and CD57 + of CD28 −: B = 1.02, 95% CI = 0.38-1.66, P =.002) and increased glycolysis (B = 0.97; 95% CI, 0.27-1.67; P =.007). Risk behavior was associated with increase activation of CD4 + T cells (B = 0.22; 95% CI, 0.07-0.37; P =.005), increased terminal differentiation of CD4 + (B = 0.82; 95% CI, 0.44-1.20; P <.0001) and CD8 + T cells (B = 1.55; 95% CI, 0.58-2.51; P =.002), and decreased glycolysis (glycolysis: B = −0.40, 95% CI = −0.68 to 0.12, P =.006; and glycolytic capacity: B = −0.54, 95% CI = −0.91 to 0.16, P =.005). Conclusions. Men who have sex with men show an increased prevalence of bloodborne and sexually transmitted infection, indicating that immunological changes in the T-cell population and the bioenergy metabolism observed in MSM can most likely be attributed to chronic antigen exposure.
KW - Bioenergy
KW - CMV
KW - MSM
KW - Risk behavior
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=85092117270&partnerID=8YFLogxK
U2 - https://doi.org/10.1093/ofid/ofaa284
DO - https://doi.org/10.1093/ofid/ofaa284
M3 - Article
C2 - 32782910
SN - 2328-8957
VL - 7
JO - Open forum infectious diseases
JF - Open forum infectious diseases
IS - 8
M1 - ofaa284
ER -