TY - JOUR
T1 - Human mast cells capture, store, and release bioactive, exogenous IL-17A
AU - Noordenbos, Troy
AU - Blijdorp, Iris
AU - Chen, Sijia
AU - Stap, Jan
AU - Mul, Erik
AU - Cañete, Juan D.
AU - Lubberts, Erik
AU - Yeremenko, Nataliya
AU - Baeten, Dominique
PY - 2016
Y1 - 2016
N2 - Mast cells cannot produce, but take up exogenous IL-17A. IL-17A, a major proinflammatory cytokine, can be produced by a variety of leukocytes, but its exact cellular source in human inflammatory diseases remains incompletely understood. IL-17A protein is abundantly found in mast cells in human tissues, such as inflamed synovium, but surprisingly, mechanistic murine studies failed to demonstrate IL-17A production by mast cells. Here, we demonstrate that primary human tissue mast cells do not produce IL-17A themselves but actively capture exogenous IL-17A through receptor-mediated endocytosis. The exogenous IL-17A is stored in intracellular granules and can subsequently be released in a bioactive form. This novel mechanism confers to mast cells the capacity to steer IL-17A-mediated tissue inflammation by the rapid release of preformed cytokine
AB - Mast cells cannot produce, but take up exogenous IL-17A. IL-17A, a major proinflammatory cytokine, can be produced by a variety of leukocytes, but its exact cellular source in human inflammatory diseases remains incompletely understood. IL-17A protein is abundantly found in mast cells in human tissues, such as inflamed synovium, but surprisingly, mechanistic murine studies failed to demonstrate IL-17A production by mast cells. Here, we demonstrate that primary human tissue mast cells do not produce IL-17A themselves but actively capture exogenous IL-17A through receptor-mediated endocytosis. The exogenous IL-17A is stored in intracellular granules and can subsequently be released in a bioactive form. This novel mechanism confers to mast cells the capacity to steer IL-17A-mediated tissue inflammation by the rapid release of preformed cytokine
U2 - https://doi.org/10.1189/jlb.3HI1215-542R
DO - https://doi.org/10.1189/jlb.3HI1215-542R
M3 - Article
C2 - 27034403
SN - 0741-5400
VL - 100
SP - 453
EP - 462
JO - Journal of leukocyte biology
JF - Journal of leukocyte biology
IS - 3
ER -