Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei

Rosalina M.L. Van Spaendonk; Glenn A. McConkey; Jai Ramesar; Andrei Gabrielian; Thomas F. McCutchan; Chris J. Janse; Andrew P. Waters

doi:https://doi.org/10.1016/S0166-6851(99)00016-X

Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei

Rosalina M.L. Van Spaendonk, Glenn A. McConkey, Jai Ramesar, Andrei Gabrielian, Thomas F. McCutchan, Chris J. Janse, Andrew P. Waters

Human Genetics (VUmc)

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

The start site of the A-type ribosomal RNA transcription units of the rodent malaria parasite, Plasmodium berghei, has been identified. The two A-type units cannot be distinguished within the transcription unit, yet exist as single copies on different chromosomes. Gene transcription initiates 820 bp upstream of the A-type small subunit (SSU) ribosomal gene and two major processing sites were mapped 610 and 611 nucleotides upstream of the SSU in the external transcribed spacer region. Surprisingly the nucleotide sequence of the DNA region containing the putative ribosomal promoter lacked repetitive DNA sequences typical of ribosomal promoters. This region was further analysed by computer using programs designed to reveal sequence-dependent structural features. Comparison of DNA curvature, duplex stability and pattern of twist angle variation revealed a striking degree of conservation between the ribosomal promoters from Plasmodium and other eukaryotes. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	193-205
Number of pages	13
Journal	Molecular and Biochemical Parasitology
Volume	99
Issue number	2
DOIs	https://doi.org/10.1016/S0166-6851(99)00016-X
Publication status	Published - 30 Apr 1999

Keywords

Malaria
Plasmodium berghei
Ribosomal RNA
Transcription

Access to Document

https://doi.org/10.1016/S0166-6851(99)00016-X

Cite this

@article{4083759dad67419c9e8483920408ed9c,

title = "Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei",

abstract = "The start site of the A-type ribosomal RNA transcription units of the rodent malaria parasite, Plasmodium berghei, has been identified. The two A-type units cannot be distinguished within the transcription unit, yet exist as single copies on different chromosomes. Gene transcription initiates 820 bp upstream of the A-type small subunit (SSU) ribosomal gene and two major processing sites were mapped 610 and 611 nucleotides upstream of the SSU in the external transcribed spacer region. Surprisingly the nucleotide sequence of the DNA region containing the putative ribosomal promoter lacked repetitive DNA sequences typical of ribosomal promoters. This region was further analysed by computer using programs designed to reveal sequence-dependent structural features. Comparison of DNA curvature, duplex stability and pattern of twist angle variation revealed a striking degree of conservation between the ribosomal promoters from Plasmodium and other eukaryotes. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Malaria, Plasmodium berghei, Ribosomal RNA, Transcription",

author = "{Van Spaendonk}, {Rosalina M.L.} and McConkey, {Glenn A.} and Jai Ramesar and Andrei Gabrielian and McCutchan, {Thomas F.} and Janse, {Chris J.} and Waters, {Andrew P.}",

note = "Funding Information: This research was supported by both the Research Council for Earth and Life Sciences (ALW), subsidised by the Netherlands Organisation for Scientific Research (NWO), and the INDO-DC programme of the European Community (Contract CT 960052).",

year = "1999",

month = apr,

day = "30",

doi = "https://doi.org/10.1016/S0166-6851(99)00016-X",

language = "English",

volume = "99",

pages = "193--205",

journal = "Molecular and Biochemical Parasitology",

issn = "0166-6851",

publisher = "Elsevier",

number = "2",

}

Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei. / Van Spaendonk, Rosalina M.L.; McConkey, Glenn A.; Ramesar, Jai et al.
In: Molecular and Biochemical Parasitology, Vol. 99, No. 2, 30.04.1999, p. 193-205.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei

AU - Van Spaendonk, Rosalina M.L.

AU - McConkey, Glenn A.

AU - Ramesar, Jai

AU - Gabrielian, Andrei

AU - McCutchan, Thomas F.

AU - Janse, Chris J.

AU - Waters, Andrew P.

N1 - Funding Information: This research was supported by both the Research Council for Earth and Life Sciences (ALW), subsidised by the Netherlands Organisation for Scientific Research (NWO), and the INDO-DC programme of the European Community (Contract CT 960052).

PY - 1999/4/30

Y1 - 1999/4/30

N2 - The start site of the A-type ribosomal RNA transcription units of the rodent malaria parasite, Plasmodium berghei, has been identified. The two A-type units cannot be distinguished within the transcription unit, yet exist as single copies on different chromosomes. Gene transcription initiates 820 bp upstream of the A-type small subunit (SSU) ribosomal gene and two major processing sites were mapped 610 and 611 nucleotides upstream of the SSU in the external transcribed spacer region. Surprisingly the nucleotide sequence of the DNA region containing the putative ribosomal promoter lacked repetitive DNA sequences typical of ribosomal promoters. This region was further analysed by computer using programs designed to reveal sequence-dependent structural features. Comparison of DNA curvature, duplex stability and pattern of twist angle variation revealed a striking degree of conservation between the ribosomal promoters from Plasmodium and other eukaryotes. Copyright (C) 1999 Elsevier Science B.V.

AB - The start site of the A-type ribosomal RNA transcription units of the rodent malaria parasite, Plasmodium berghei, has been identified. The two A-type units cannot be distinguished within the transcription unit, yet exist as single copies on different chromosomes. Gene transcription initiates 820 bp upstream of the A-type small subunit (SSU) ribosomal gene and two major processing sites were mapped 610 and 611 nucleotides upstream of the SSU in the external transcribed spacer region. Surprisingly the nucleotide sequence of the DNA region containing the putative ribosomal promoter lacked repetitive DNA sequences typical of ribosomal promoters. This region was further analysed by computer using programs designed to reveal sequence-dependent structural features. Comparison of DNA curvature, duplex stability and pattern of twist angle variation revealed a striking degree of conservation between the ribosomal promoters from Plasmodium and other eukaryotes. Copyright (C) 1999 Elsevier Science B.V.

KW - Malaria

KW - Plasmodium berghei

KW - Ribosomal RNA

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0032910976&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/S0166-6851(99)00016-X

DO - https://doi.org/10.1016/S0166-6851(99)00016-X

M3 - Article

C2 - 10340484

SN - 0166-6851

VL - 99

SP - 193

EP - 205

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 2

ER -

Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei

Abstract

Keywords

Access to Document

Other files and links

Cite this