TY - JOUR
T1 - Immaturity of human stem-cell-derived cardiomyocytes in culture: fatal flaw or soluble problem?
AU - Veerman, Christiaan C.
AU - Kosmidis, Georgios
AU - Mummery, Christine L.
AU - Casini, Simona
AU - Verkerk, Arie O.
AU - Bellin, Milena
PY - 2015
Y1 - 2015
N2 - Cardiomyocytes from human pluripotent stem cells (hPSC-CMs) are increasingly used to model cardiac disease, test drug efficacy and for safety pharmacology. Nevertheless, a major hurdle to more extensive use is their immaturity and similarity to fetal rather than adult cardiomyocytes. Here, we provide an overview of the strategies currently being used to increase maturation in culture, which include prolongation of time in culture, exposure to electrical stimulation, application of mechanical strain, growth in three-dimensional tissue configuration, addition of non-cardiomyocytes, use of hormones and small molecules, and alteration of the extracellular environment. By comparing the outcomes of these studies, we identify the approaches most likely to improve functional maturation of hPSC-CMs in terms of their electrophysiology and excitation-contraction coupling
AB - Cardiomyocytes from human pluripotent stem cells (hPSC-CMs) are increasingly used to model cardiac disease, test drug efficacy and for safety pharmacology. Nevertheless, a major hurdle to more extensive use is their immaturity and similarity to fetal rather than adult cardiomyocytes. Here, we provide an overview of the strategies currently being used to increase maturation in culture, which include prolongation of time in culture, exposure to electrical stimulation, application of mechanical strain, growth in three-dimensional tissue configuration, addition of non-cardiomyocytes, use of hormones and small molecules, and alteration of the extracellular environment. By comparing the outcomes of these studies, we identify the approaches most likely to improve functional maturation of hPSC-CMs in terms of their electrophysiology and excitation-contraction coupling
U2 - https://doi.org/10.1089/scd.2014.0533
DO - https://doi.org/10.1089/scd.2014.0533
M3 - Review article
C2 - 25583389
SN - 1547-3287
VL - 24
SP - 1035
EP - 1052
JO - Stem cells and development
JF - Stem cells and development
IS - 9
ER -