Improved rat liver preservation by hypothermic continuous machine perfusion using Polysol, a new, enriched preservation solution

Maud Bessems, Benedict M. Doorschodt, Arlène K. van Vliet, Thomas M. van Gulik

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

For experimental machine perfusion (MP) of the liver, the modified University of Wisconsin solution (UW-G) is most often used. In our search for an enriched NIP preservation solution, Polysol was developed. Polysol is enriched with various amino acids, vitamins, and other nutrients for the liver metabolism. The aim of this study was to compare Polysol with UW-G for MP preservation of the liver. Rat livers were preserved during 24 hours with hypothermic NIP using UW-G (n = 5) or Polysol (n = 5). Hepatocellular damage (aspartate aminotransferase [AST], alanine aminotransferase [ALT], lactate dehydrogcnase [LDH], alpha-glutathione-S-transferase [alpha-GST]) and bile production were measured during 60 minutes of reperfusion (370 with Krebs-Henseleit buffer. Control livers were reperfused after 24 hours of cold storage in UW (n = 5). NIP using UW-G or Polysol showed less liver damage when compared with controls. Livers machine perfuscd with Polysol showed less enzyme release when compared to UW-G. Bile production was higher after NIP using either UW-G or Polysol compared with controls. In conclusion, machine perfusion using Polysol results in better quality liver preservation than cold storage with UW and machine perfusion using UW-G
Original languageEnglish
Pages (from-to)539-546
JournalLiver transplantation
Volume11
Issue number5
DOIs
Publication statusPublished - 2005

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