In situ-noninvasive electrochemical monitoring of microtissue differentiation in microwell arrays

A. Sridhar, A. Van Den Berg, S. Le Gac

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We report a non-invasive approach using scanning electrochemical microscopy for monitoring microtissue differentiation through the indirect detection of alkaline phosphatase. C2C12 microtissues produced in microwell arrays - stamped in commercially available polystyrene Petri dishes- are treated with 300 ng/mL bone morphogenic protein 2 to induce their differentiation into osteoblasts. Alkaline phosphatase in differentiated cells converts an electrochemically inactive substrate into an electrochemically active product, which is subsequently oxidized in the tissue vicinity using the SECM tip. Thereby, tissue differentiation can be monitored in a non-invasive and continuous way in live microtissues, while eliminating tedious and time-consuming preparation and cell fixation.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages254-256
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/201331/10/2013

Keywords

  • Alkaline phosphatase
  • Differentiation assay
  • Secm
  • Stamped petri dishes

Cite this