TY - JOUR
T1 - In vitro evaluation of traditionally used Surinamese medicinal plants for their potential anti-leishmanial efficacy
AU - Mans, D. R. A.
AU - Beerens, T.
AU - Magali, I.
AU - Soekhoe, R. C.
AU - Schoone, G. J.
AU - Oedairadjsingh, K.
AU - Hasrat, J. A.
AU - van den Bogaart, E.
AU - Schallig, H. D. F. H.
PY - 2016
Y1 - 2016
N2 - Plant-based preparations are extensively used in Surinamese folk medicine for treating leishmaniasis, but often without a scientific rationale. To evaluate 25 Surinamese medicinal plants for their potential efficacy against leishmaniasis. Concentrated plant extracts were evaluated for their effect on the viability of L. (V.) guyanensis AMC, L. (L.) major NADIM5, and L. (L.) donovani GEDII promastigotes, as well as intracellular amastigotes of L. (L.) donovani BHU814 in infected THP-1 cells. Selectivity was assessed by cytotoxicity against THP-1 cells. The only plant extract that showed potentially meaningful anti-leishmanial activity was that from Solanum lycocarpum that displayed mean IC50 values of about 51, 61, and <16µg/mL against L. (V) guyanensis, L. (L) major, and L. (L) donovani promastigotes, respectively; about 374µg/mL against L. (L) donovani amastigotes; and >500µg/mL against THP-1 cells. The Bryophyllum pinnatum, Inga alba, and Quassia amara extracts displayed moderate to high IC50 values against promastigotes (about 51 to >500µg/mL) and/or amastigotes (about 224 to >500µg/mL) but were relatively toxic to THP-1 cells (IC50 values <16 to about 42µg/mL). The remaining plant extracts exhibited in many cases IC50 values close to, around, or above 500µg/mL against promastigotes, amastigotes, and THP-1 cells. The S. lycocarpum preparation may be useful against leishmaniasis and may have a good safety index, warranting further investigations into its active constituents and mechanism(s) of action
AB - Plant-based preparations are extensively used in Surinamese folk medicine for treating leishmaniasis, but often without a scientific rationale. To evaluate 25 Surinamese medicinal plants for their potential efficacy against leishmaniasis. Concentrated plant extracts were evaluated for their effect on the viability of L. (V.) guyanensis AMC, L. (L.) major NADIM5, and L. (L.) donovani GEDII promastigotes, as well as intracellular amastigotes of L. (L.) donovani BHU814 in infected THP-1 cells. Selectivity was assessed by cytotoxicity against THP-1 cells. The only plant extract that showed potentially meaningful anti-leishmanial activity was that from Solanum lycocarpum that displayed mean IC50 values of about 51, 61, and <16µg/mL against L. (V) guyanensis, L. (L) major, and L. (L) donovani promastigotes, respectively; about 374µg/mL against L. (L) donovani amastigotes; and >500µg/mL against THP-1 cells. The Bryophyllum pinnatum, Inga alba, and Quassia amara extracts displayed moderate to high IC50 values against promastigotes (about 51 to >500µg/mL) and/or amastigotes (about 224 to >500µg/mL) but were relatively toxic to THP-1 cells (IC50 values <16 to about 42µg/mL). The remaining plant extracts exhibited in many cases IC50 values close to, around, or above 500µg/mL against promastigotes, amastigotes, and THP-1 cells. The S. lycocarpum preparation may be useful against leishmaniasis and may have a good safety index, warranting further investigations into its active constituents and mechanism(s) of action
U2 - https://doi.org/10.1016/j.jep.2016.01.012
DO - https://doi.org/10.1016/j.jep.2016.01.012
M3 - Article
C2 - 26778603
SN - 0378-8741
VL - 180
SP - 70
EP - 77
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -