In Vitro Meiosis of Male Germline Stem Cells

Qijing Lei; Xin Lai; Jitske Eliveld; Susana M. Chuva de Sousa Lopes; Ans M. M. van Pelt; Geert Hamer

doi:https://doi.org/10.1016/j.stemcr.2020.10.006

In Vitro Meiosis of Male Germline Stem Cells

Qijing Lei, Xin Lai, Jitske Eliveld, Susana M. Chuva de Sousa Lopes, Ans M. M. van Pelt, Geert Hamer

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

In this article, Hamer and colleagues show that, on a feeder layer of Sertoli cells, mouse spermatogonial stem cells can be induced to undergo meiosis in vitro. However, meiotic recombination and checkpoints do not necessarily function properly in vitro, and the authors warn that in vitro-generated spermatid-like cells should be thoroughly analyzed before clinical application can be considered.

Original language	English
Pages (from-to)	1140-1153
Number of pages	14
Journal	Stem cell reports
Volume	15
Issue number	5
DOIs	https://doi.org/10.1016/j.stemcr.2020.10.006
Publication status	Published - 10 Nov 2020

Keywords

Sertoli cell lines
in vitro spermatogenesis
meiosis
meiotic checkpoints
spermatogonial stem cells

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https://doi.org/10.1016/j.stemcr.2020.10.006

Cite this

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title = "In Vitro Meiosis of Male Germline Stem Cells",

abstract = "In this article, Hamer and colleagues show that, on a feeder layer of Sertoli cells, mouse spermatogonial stem cells can be induced to undergo meiosis in vitro. However, meiotic recombination and checkpoints do not necessarily function properly in vitro, and the authors warn that in vitro-generated spermatid-like cells should be thoroughly analyzed before clinical application can be considered.",

keywords = "Sertoli cell lines, in vitro spermatogenesis, meiosis, meiotic checkpoints, spermatogonial stem cells",

author = "Qijing Lei and Xin Lai and Jitske Eliveld and {Chuva de Sousa Lopes}, {Susana M.} and {van Pelt}, {Ans M. M.} and Geert Hamer",

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T1 - In Vitro Meiosis of Male Germline Stem Cells

AU - Lei, Qijing

AU - Lai, Xin

AU - Eliveld, Jitske

AU - Chuva de Sousa Lopes, Susana M.

AU - van Pelt, Ans M. M.

AU - Hamer, Geert

PY - 2020/11/10

Y1 - 2020/11/10

N2 - In this article, Hamer and colleagues show that, on a feeder layer of Sertoli cells, mouse spermatogonial stem cells can be induced to undergo meiosis in vitro. However, meiotic recombination and checkpoints do not necessarily function properly in vitro, and the authors warn that in vitro-generated spermatid-like cells should be thoroughly analyzed before clinical application can be considered.

AB - In this article, Hamer and colleagues show that, on a feeder layer of Sertoli cells, mouse spermatogonial stem cells can be induced to undergo meiosis in vitro. However, meiotic recombination and checkpoints do not necessarily function properly in vitro, and the authors warn that in vitro-generated spermatid-like cells should be thoroughly analyzed before clinical application can be considered.

KW - Sertoli cell lines

KW - in vitro spermatogenesis

KW - meiosis

KW - meiotic checkpoints

KW - spermatogonial stem cells

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U2 - https://doi.org/10.1016/j.stemcr.2020.10.006

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JO - Stem cell reports

JF - Stem cell reports

IS - 5

ER -

In Vitro Meiosis of Male Germline Stem Cells

Abstract

Keywords

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