In Vivo Models of Mechanical Loading

Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, Andrew A. Pitsillides

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)


The skeleton fulfils its mechanical functions through structural organization and material properties of individual bones. It is stated that both cortical and trabecular morphology and mass can be (re)modelled in response to changes in mechanical strains engendered by load-bearing. To address this, animal models that enable the application of specific loads to individual bones have been developed. These are useful in defining how loading modulates (re)modeling and allow examination of the mechanisms that coordinate these events. This chapter describes how to apply mechanical loading to murine bones through points of articulation, which allows changes in endosteal, periosteal as well as trabecular bone to be revealed at multiple hierarchies, by a host of methodologies, including double fluorochrome labeling and computed tomography.
Original languageEnglish
Pages (from-to)369-390
Number of pages22
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 1 Jan 2019


  • Adaptation
  • Cancellous bone
  • Cortical bone
  • Mechanical loading
  • Mouse

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