Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria

Jochem H. Bernink; Lisette Krabbendam; Kristine Germar; Esther de Jong; Konrad Gronke; Michael Kofoed-Nielsen; J. Marius Munneke; Mette D. Hazenberg; Julien Villaudy; Christianne J. Buskens; Willem A. Bemelman; Andreas Diefenbach; Bianca Blom; Hergen Spits

doi:https://doi.org/10.1016/j.immuni.2015.06.019

Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria

Jochem H. Bernink, Lisette Krabbendam, Kristine Germar, Esther de Jong, Konrad Gronke, Michael Kofoed-Nielsen, J. Marius Munneke, Mette D. Hazenberg, Julien Villaudy, Christianne J. Buskens, Willem A. Bemelman, Andreas Diefenbach, Bianca Blom, Hergen Spits

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Human group 1 ILCs consist of at least three phenotypically distinct subsets, including NK cells, CD127(+) ILC1, and intraepithelial CD103(+) ILC1. In inflamed intestinal tissues from Crohn's disease patients, numbers of CD127(+) ILC1 increased at the cost of ILC3. Here we found that differentiation of ILC3 to CD127(+) ILC1 is reversible in vitro and in vivo. CD127(+) ILC1 differentiated to ILC3 in the presence of interleukin-2 (IL-2), IL-23, and IL-1β dependent on the transcription factor RORγt, and this process was enhanced in the presence of retinoic acid. Furthermore, we observed in resection specimen from Crohn's disease patients a higher proportion of CD14(+) dendritic cells (DC), which in vitro promoted polarization from ILC3 to CD127(+) ILC1. In contrast, CD14(-) DCs promoted differentiation from CD127(+) ILC1 toward ILC3. These observations suggest that environmental cues determine the composition, function, and phenotype of CD127(+) ILC1 and ILC3 in the gut

Original language	English
Pages (from-to)	146-160
Journal	Immunity
Volume	43
Issue number	1
DOIs	https://doi.org/10.1016/j.immuni.2015.06.019
Publication status	Published - 2015

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https://doi.org/10.1016/j.immuni.2015.06.019

Cite this

Bernink, J. H., Krabbendam, L., Germar, K., de Jong, E., Gronke, K., Kofoed-Nielsen, M., Munneke, J. M., Hazenberg, M. D., Villaudy, J., Buskens, C. J., Bemelman, W. A., Diefenbach, A., Blom, B., & Spits, H. (2015). Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria. Immunity, 43(1), 146-160. https://doi.org/10.1016/j.immuni.2015.06.019

@article{45520b5e06974289bc8807d72421160a,

title = "Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria",

abstract = "Human group 1 ILCs consist of at least three phenotypically distinct subsets, including NK cells, CD127(+) ILC1, and intraepithelial CD103(+) ILC1. In inflamed intestinal tissues from Crohn's disease patients, numbers of CD127(+) ILC1 increased at the cost of ILC3. Here we found that differentiation of ILC3 to CD127(+) ILC1 is reversible in vitro and in vivo. CD127(+) ILC1 differentiated to ILC3 in the presence of interleukin-2 (IL-2), IL-23, and IL-1β dependent on the transcription factor RORγt, and this process was enhanced in the presence of retinoic acid. Furthermore, we observed in resection specimen from Crohn's disease patients a higher proportion of CD14(+) dendritic cells (DC), which in vitro promoted polarization from ILC3 to CD127(+) ILC1. In contrast, CD14(-) DCs promoted differentiation from CD127(+) ILC1 toward ILC3. These observations suggest that environmental cues determine the composition, function, and phenotype of CD127(+) ILC1 and ILC3 in the gut",

author = "Bernink, {Jochem H.} and Lisette Krabbendam and Kristine Germar and {de Jong}, Esther and Konrad Gronke and Michael Kofoed-Nielsen and Munneke, {J. Marius} and Hazenberg, {Mette D.} and Julien Villaudy and Buskens, {Christianne J.} and Bemelman, {Willem A.} and Andreas Diefenbach and Bianca Blom and Hergen Spits",

year = "2015",

doi = "https://doi.org/10.1016/j.immuni.2015.06.019",

language = "English",

volume = "43",

pages = "146--160",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "1",

}

Bernink, JH, Krabbendam, L, Germar, K, de Jong, E, Gronke, K, Kofoed-Nielsen, M, Munneke, JM, Hazenberg, MD, Villaudy, J, Buskens, CJ , Bemelman, WA, Diefenbach, A, Blom, B & Spits, H 2015, 'Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria', Immunity, vol. 43, no. 1, pp. 146-160. https://doi.org/10.1016/j.immuni.2015.06.019

TY - JOUR

T1 - Interleukin-12 and -23 Control Plasticity of CD127(+) Group 1 and Group 3 Innate Lymphoid Cells in the Intestinal Lamina Propria

AU - Bernink, Jochem H.

AU - Krabbendam, Lisette

AU - Germar, Kristine

AU - de Jong, Esther

AU - Gronke, Konrad

AU - Kofoed-Nielsen, Michael

AU - Munneke, J. Marius

AU - Hazenberg, Mette D.

AU - Villaudy, Julien

AU - Buskens, Christianne J.

AU - Bemelman, Willem A.

AU - Diefenbach, Andreas

AU - Blom, Bianca

AU - Spits, Hergen

PY - 2015

Y1 - 2015

N2 - Human group 1 ILCs consist of at least three phenotypically distinct subsets, including NK cells, CD127(+) ILC1, and intraepithelial CD103(+) ILC1. In inflamed intestinal tissues from Crohn's disease patients, numbers of CD127(+) ILC1 increased at the cost of ILC3. Here we found that differentiation of ILC3 to CD127(+) ILC1 is reversible in vitro and in vivo. CD127(+) ILC1 differentiated to ILC3 in the presence of interleukin-2 (IL-2), IL-23, and IL-1β dependent on the transcription factor RORγt, and this process was enhanced in the presence of retinoic acid. Furthermore, we observed in resection specimen from Crohn's disease patients a higher proportion of CD14(+) dendritic cells (DC), which in vitro promoted polarization from ILC3 to CD127(+) ILC1. In contrast, CD14(-) DCs promoted differentiation from CD127(+) ILC1 toward ILC3. These observations suggest that environmental cues determine the composition, function, and phenotype of CD127(+) ILC1 and ILC3 in the gut

AB - Human group 1 ILCs consist of at least three phenotypically distinct subsets, including NK cells, CD127(+) ILC1, and intraepithelial CD103(+) ILC1. In inflamed intestinal tissues from Crohn's disease patients, numbers of CD127(+) ILC1 increased at the cost of ILC3. Here we found that differentiation of ILC3 to CD127(+) ILC1 is reversible in vitro and in vivo. CD127(+) ILC1 differentiated to ILC3 in the presence of interleukin-2 (IL-2), IL-23, and IL-1β dependent on the transcription factor RORγt, and this process was enhanced in the presence of retinoic acid. Furthermore, we observed in resection specimen from Crohn's disease patients a higher proportion of CD14(+) dendritic cells (DC), which in vitro promoted polarization from ILC3 to CD127(+) ILC1. In contrast, CD14(-) DCs promoted differentiation from CD127(+) ILC1 toward ILC3. These observations suggest that environmental cues determine the composition, function, and phenotype of CD127(+) ILC1 and ILC3 in the gut

U2 - https://doi.org/10.1016/j.immuni.2015.06.019

DO - https://doi.org/10.1016/j.immuni.2015.06.019

M3 - Article

C2 - 26187413

SN - 1074-7613

VL - 43

SP - 146

EP - 160

JO - Immunity

JF - Immunity

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ER -