TY - JOUR
T1 - Isoforms of cytochrome c oxidase in tissues and cell lines of the mouse
AU - van den Bogert, C.
AU - Dekker, H. L.
AU - Cornelissen, J. C.
AU - van Kuilenburg, A. B.
AU - Bolhuis, P. A.
AU - Muijsers, A. O.
PY - 1992
Y1 - 1992
N2 - The subunit pattern of immunopurified cytochrome c oxidase from cultured mouse cells and mature tissues of the mouse was investigated by electrophoretic analysis. In mature tissues two forms of cytochrome c oxidase could clearly be identified on the basis of differences in morbidity or staining intensity of subunits VIa and VIII. One form was present in muscle and heart, and the other in liver, kidney and spleen. In lung both forms were found. In the thymus, subunit VIII showed the characteristics of subunit VIII found in muscle and heart, whereas subunit VIa resembled subunit VIa found in liver. This suggest the existence of a third cytochrome c oxidase isoform. The subunits of cytochrome c oxidase from cultured cell lines showed no differences between the various cell lines and resembled those of mature mouse liver tissue. The cytochrome c oxidase isoform from cultured proliferating cells might therefore be the same as the one found in liver. Alternatively, it might represent either a normally occurring fetal isoform, or a form specific for poorly differentiated cultured cells
AB - The subunit pattern of immunopurified cytochrome c oxidase from cultured mouse cells and mature tissues of the mouse was investigated by electrophoretic analysis. In mature tissues two forms of cytochrome c oxidase could clearly be identified on the basis of differences in morbidity or staining intensity of subunits VIa and VIII. One form was present in muscle and heart, and the other in liver, kidney and spleen. In lung both forms were found. In the thymus, subunit VIII showed the characteristics of subunit VIII found in muscle and heart, whereas subunit VIa resembled subunit VIa found in liver. This suggest the existence of a third cytochrome c oxidase isoform. The subunits of cytochrome c oxidase from cultured cell lines showed no differences between the various cell lines and resembled those of mature mouse liver tissue. The cytochrome c oxidase isoform from cultured proliferating cells might therefore be the same as the one found in liver. Alternatively, it might represent either a normally occurring fetal isoform, or a form specific for poorly differentiated cultured cells
U2 - https://doi.org/10.1016/0005-2728(92)90207-I
DO - https://doi.org/10.1016/0005-2728(92)90207-I
M3 - Article
C2 - 1311949
SN - 0005-2728
VL - 1099
SP - 118
EP - 122
JO - BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
JF - BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
IS - 2
ER -