Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection

Winston Cavert; Daan W. Notermans; Katherine Staskus; Stephen W. Wietgrefe; Mary Zupancic; Kristin Gebhard; Keith Henry; Zhi Qiang Zhang; Roger Mills; Hugh McDade; Jaap Goudsmit; Sven A. Danner; Ashley T. Haase; C. M. Schuwirth; N. Zupancic

doi:https://doi.org/10.1126/science.276.5314.960

Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection

Winston Cavert, Daan W. Notermans, Katherine Staskus, Stephen W. Wietgrefe, Mary Zupancic, Kristin Gebhard, Keith Henry, Zhi Qiang Zhang, Roger Mills, Hugh McDade, Jaap Goudsmit, Sven A. Danner, Ashley T. Haase, C. M. Schuwirth, N. Zupancic

Research output: Contribution to journal › Article › Academic › peer-review

512 Citations (Scopus)

Abstract

In lymphoid tissue, where human immunodeficiency virus-type 1 (HIV-1) is produced and stored, three-drug treatment with viral protease and reverse transcriptase inhibitors markedly reduced viral burden. This was shown by in situ hybridization and computerized quantitative analysis of serial tonsil biopsies from previously untreated adults. The frequency of productive mononuclear cells (MNCs) initially diminished with a half-life of about 1 day. Surprisingly, the amount of HIV-1 RNA in virus trapped on follicular dendritic cells (FDCs) decreased almost as quickly. After 24 weeks, MNCs with very few copies of HIV-1 RNA per cell were still detectable, as was proviral DNA; however, the amount of FDC-associated virus decreased by ≤3.4 log units. Thus, 6 months of potent therapy controlled active replication and cleared >99.9 percent of virus from the secondary lymphoid tissue reservoir.

Original language	English
Pages (from-to)	960-964
Number of pages	5
Journal	Science
Volume	276
Issue number	5314
DOIs	https://doi.org/10.1126/science.276.5314.960
Publication status	Published - 9 May 1997

Keywords

AMC wi-buiten

Access to Document

https://doi.org/10.1126/science.276.5314.960

https://pure.uva.nl/ws/files/3043221/2795_26661y.pdf

Cite this

Cavert, W., Notermans, D. W., Staskus, K., Wietgrefe, S. W., Zupancic, M., Gebhard, K., Henry, K., Zhang, Z. Q., Mills, R., McDade, H., Goudsmit, J., Danner, S. A., Haase, A. T., Schuwirth, C. M., & Zupancic, N. (1997). Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection. Science, 276(5314), 960-964. https://doi.org/10.1126/science.276.5314.960

@article{b6ecfef4b2524474b3ff415651333e3f,

title = "Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection",

abstract = "In lymphoid tissue, where human immunodeficiency virus-type 1 (HIV-1) is produced and stored, three-drug treatment with viral protease and reverse transcriptase inhibitors markedly reduced viral burden. This was shown by in situ hybridization and computerized quantitative analysis of serial tonsil biopsies from previously untreated adults. The frequency of productive mononuclear cells (MNCs) initially diminished with a half-life of about 1 day. Surprisingly, the amount of HIV-1 RNA in virus trapped on follicular dendritic cells (FDCs) decreased almost as quickly. After 24 weeks, MNCs with very few copies of HIV-1 RNA per cell were still detectable, as was proviral DNA; however, the amount of FDC-associated virus decreased by ≤3.4 log units. Thus, 6 months of potent therapy controlled active replication and cleared >99.9 percent of virus from the secondary lymphoid tissue reservoir.",

keywords = "AMC wi-buiten",

author = "Winston Cavert and Notermans, {Daan W.} and Katherine Staskus and Wietgrefe, {Stephen W.} and Mary Zupancic and Kristin Gebhard and Keith Henry and Zhang, {Zhi Qiang} and Roger Mills and Hugh McDade and Jaap Goudsmit and Danner, {Sven A.} and Haase, {Ashley T.} and Schuwirth, {C. M.} and N. Zupancic",

note = "USA",

year = "1997",

month = may,

day = "9",

doi = "https://doi.org/10.1126/science.276.5314.960",

language = "English",

volume = "276",

pages = "960--964",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5314",

}

TY - JOUR

T1 - Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection

AU - Cavert, Winston

AU - Notermans, Daan W.

AU - Staskus, Katherine

AU - Wietgrefe, Stephen W.

AU - Zupancic, Mary

AU - Gebhard, Kristin

AU - Henry, Keith

AU - Zhang, Zhi Qiang

AU - Mills, Roger

AU - McDade, Hugh

AU - Goudsmit, Jaap

AU - Danner, Sven A.

AU - Haase, Ashley T.

AU - Schuwirth, C. M.

AU - Zupancic, N.

N1 - USA

PY - 1997/5/9

Y1 - 1997/5/9

N2 - In lymphoid tissue, where human immunodeficiency virus-type 1 (HIV-1) is produced and stored, three-drug treatment with viral protease and reverse transcriptase inhibitors markedly reduced viral burden. This was shown by in situ hybridization and computerized quantitative analysis of serial tonsil biopsies from previously untreated adults. The frequency of productive mononuclear cells (MNCs) initially diminished with a half-life of about 1 day. Surprisingly, the amount of HIV-1 RNA in virus trapped on follicular dendritic cells (FDCs) decreased almost as quickly. After 24 weeks, MNCs with very few copies of HIV-1 RNA per cell were still detectable, as was proviral DNA; however, the amount of FDC-associated virus decreased by ≤3.4 log units. Thus, 6 months of potent therapy controlled active replication and cleared >99.9 percent of virus from the secondary lymphoid tissue reservoir.

AB - In lymphoid tissue, where human immunodeficiency virus-type 1 (HIV-1) is produced and stored, three-drug treatment with viral protease and reverse transcriptase inhibitors markedly reduced viral burden. This was shown by in situ hybridization and computerized quantitative analysis of serial tonsil biopsies from previously untreated adults. The frequency of productive mononuclear cells (MNCs) initially diminished with a half-life of about 1 day. Surprisingly, the amount of HIV-1 RNA in virus trapped on follicular dendritic cells (FDCs) decreased almost as quickly. After 24 weeks, MNCs with very few copies of HIV-1 RNA per cell were still detectable, as was proviral DNA; however, the amount of FDC-associated virus decreased by ≤3.4 log units. Thus, 6 months of potent therapy controlled active replication and cleared >99.9 percent of virus from the secondary lymphoid tissue reservoir.

KW - AMC wi-buiten

UR - http://www.scopus.com/inward/record.url?scp=15144342918&partnerID=8YFLogxK

U2 - https://doi.org/10.1126/science.276.5314.960

DO - https://doi.org/10.1126/science.276.5314.960

M3 - Article

C2 - 9139661

SN - 0036-8075

VL - 276

SP - 960

EP - 964

JO - Science

JF - Science

IS - 5314

ER -

Kinetics of response in lymphoid tissues to antiretroviral therapy of HIV-1 infection

Abstract

Keywords

Access to Document

Other files and links

Cite this