Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue

Ludo van Haasterecht; Liron Zada; Robert W. Schmidt; Erik de Bakker; Ellis Barbé; Heather A. Leslie; A. Dick Vethaak; Susan Gibbs; Johannes F. de Boer; Frank B. Niessen; Paul P. M. van Zuijlen; Marie Louise Groot; Freek Ariese

doi:https://doi.org/10.1002/jbio.201960197

Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue

Ludo van Haasterecht, Liron Zada, Robert W. Schmidt, Erik de Bakker, Ellis Barbé, Heather A. Leslie, A. Dick Vethaak, Susan Gibbs, Johannes F. de Boer, Frank B. Niessen, Paul P. M. van Zuijlen, Marie Louise Groot, Freek Ariese

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright-field images revealed the distribution and quantity of silicone material in the tissue. Fast and high-resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant-linked diseases.

Original language	English
Article number	e201960197
Pages (from-to)	e201960197
Number of pages	10
Journal	Journal of Biophotonics
Volume	13
Issue number	5
Early online date	12 Feb 2020
DOIs	https://doi.org/10.1002/jbio.201960197
Publication status	Published - 1 May 2020

Keywords

capsular contraction
label-free histology
nonlinear optics
pathology
plastic surgery
polymer detection
silicone bleeding

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van Haasterecht, L., Zada, L., Schmidt, R. W., de Bakker, E., Barbé, E., Leslie, H. A., Vethaak, A. D., Gibbs, S., de Boer, J. F., Niessen, F. B., van Zuijlen, P. P. M., Groot, M. L., & Ariese, F. (2020). Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue. Journal of Biophotonics, 13(5), e201960197. Article e201960197. https://doi.org/10.1002/jbio.201960197

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title = "Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue",

abstract = "Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright-field images revealed the distribution and quantity of silicone material in the tissue. Fast and high-resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant-linked diseases.",

keywords = "capsular contraction, label-free histology, nonlinear optics, pathology, plastic surgery, polymer detection, silicone bleeding",

author = "{van Haasterecht}, Ludo and Liron Zada and Schmidt, {Robert W.} and {de Bakker}, Erik and Ellis Barb{\'e} and Leslie, {Heather A.} and Vethaak, {A. Dick} and Susan Gibbs and {de Boer}, {Johannes F.} and Niessen, {Frank B.} and {van Zuijlen}, {Paul P. M.} and Groot, {Marie Louise} and Freek Ariese",

note = "Funding Information: Thanks are due to Bram Mooij for help with exploratory Raman measurements. EU-Horizon 2020 (Laserlab Europe, Grant Number: 654148). Dutch Research Council (NWO) in the framework of COAST (Grant Number: 053.21.112). The collaboration project is cofunded by the PPP Allowance made available by Health~Holland, Top Sector Life Sciences & Health, to stimulate public?private partnerships as well as by a contribution from the Dutch Burn Foundation (grant number: TKI 16.01). Publisher Copyright: {\textcopyright} 2020 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = may,

day = "1",

doi = "https://doi.org/10.1002/jbio.201960197",

language = "English",

volume = "13",

pages = "e201960197",

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van Haasterecht, L, Zada, L, Schmidt, RW, de Bakker, E , Barbé, E, Leslie, HA, Vethaak, AD, Gibbs, S , de Boer, JF , Niessen, FB , van Zuijlen, PPM, Groot, ML & Ariese, F 2020, 'Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue', Journal of Biophotonics, vol. 13, no. 5, e201960197, pp. e201960197. https://doi.org/10.1002/jbio.201960197

TY - JOUR

T1 - Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue

AU - van Haasterecht, Ludo

AU - Zada, Liron

AU - Schmidt, Robert W.

AU - de Bakker, Erik

AU - Barbé, Ellis

AU - Leslie, Heather A.

AU - Vethaak, A. Dick

AU - Gibbs, Susan

AU - de Boer, Johannes F.

AU - Niessen, Frank B.

AU - van Zuijlen, Paul P. M.

AU - Groot, Marie Louise

AU - Ariese, Freek

N1 - Funding Information: Thanks are due to Bram Mooij for help with exploratory Raman measurements. EU-Horizon 2020 (Laserlab Europe, Grant Number: 654148). Dutch Research Council (NWO) in the framework of COAST (Grant Number: 053.21.112). The collaboration project is cofunded by the PPP Allowance made available by Health~Holland, Top Sector Life Sciences & Health, to stimulate public?private partnerships as well as by a contribution from the Dutch Burn Foundation (grant number: TKI 16.01). Publisher Copyright: © 2020 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright-field images revealed the distribution and quantity of silicone material in the tissue. Fast and high-resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant-linked diseases.

AB - Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant. SRS images coregistered with bright-field images revealed the distribution and quantity of silicone material in the tissue. Fast and high-resolution imaging of histology slides with molecular specificity using SRS provides an opportunity to investigate the role of silicone debris in the pathophysiology of implant-linked diseases.

KW - capsular contraction

KW - label-free histology

KW - nonlinear optics

KW - pathology

KW - plastic surgery

KW - polymer detection

KW - silicone bleeding

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DO - https://doi.org/10.1002/jbio.201960197

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C2 - 32049417

SN - 1864-063X

VL - 13

SP - e201960197

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 5

M1 - e201960197

ER -

Label-free stimulated Raman scattering imaging reveals silicone breast implant material in tissue

Abstract

Keywords

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