TY - JOUR
T1 - Laboratory aspects of circulating alpha-Klotho
AU - Heijboer, A.C.
AU - Blankenstein, M.A.
AU - Hoenderop, J.
AU - de Borst, M.H.
AU - Vervloet, M.G.
PY - 2013
Y1 - 2013
N2 - Background. alpha-Klotho is a protein mainly produced in the kidney. Its circulating form has been suggested to link renal damage and distant tissue pathology. As three assays to measure alpha-Klotho became commercially available, we performed an evaluation of these commercially available Klotho assays. Methods. We studied within-run variation, between-run variation, matrix effects, linearity, and recovery of added recombinant human Klotho in the alpha-Klotho assays of IBL (IBL International GmbH, Hamburg, Germany), Cusabio (Cusabio Biotech, Wuhan, China) and USCN (USCN life Science, Inc., Wuhan, China) using both serum and ethylenediaminetetraacetic acid plasma. Results. Within run variation was 4, 13 and 32% for the IBL, Cusabio and USCN assay, respectively. Agreement between serum and EDTA plasma was good in the IBL assay, but poor in the USCN and Cusabio assays however improved after modifications in the Cusabio assay. Standardization and agreement between assays was poor. Conclusions. The commercially available methods for the measurement of alpha-Klotho differ in quality. Some of the manufacturers should improve their assays in order to produce accurate results so that reliable conclusions can be drawn from studies in which these assays are used
AB - Background. alpha-Klotho is a protein mainly produced in the kidney. Its circulating form has been suggested to link renal damage and distant tissue pathology. As three assays to measure alpha-Klotho became commercially available, we performed an evaluation of these commercially available Klotho assays. Methods. We studied within-run variation, between-run variation, matrix effects, linearity, and recovery of added recombinant human Klotho in the alpha-Klotho assays of IBL (IBL International GmbH, Hamburg, Germany), Cusabio (Cusabio Biotech, Wuhan, China) and USCN (USCN life Science, Inc., Wuhan, China) using both serum and ethylenediaminetetraacetic acid plasma. Results. Within run variation was 4, 13 and 32% for the IBL, Cusabio and USCN assay, respectively. Agreement between serum and EDTA plasma was good in the IBL assay, but poor in the USCN and Cusabio assays however improved after modifications in the Cusabio assay. Standardization and agreement between assays was poor. Conclusions. The commercially available methods for the measurement of alpha-Klotho differ in quality. Some of the manufacturers should improve their assays in order to produce accurate results so that reliable conclusions can be drawn from studies in which these assays are used
U2 - https://doi.org/10.1093/ndt/gft236
DO - https://doi.org/10.1093/ndt/gft236
M3 - Article
C2 - 23787542
SN - 0931-0509
VL - 28
SP - 2283
EP - 2287
JO - Nephrology, dialysis, transplantation
JF - Nephrology, dialysis, transplantation
IS - 9
ER -