TY - JOUR
T1 - Leukocyte subpopulations and arteriogenesis: Specific role of monocytes, lymphocytes and granulocytes
AU - Hoefer, Imo E.
AU - Grundmann, Sebastian
AU - van Royen, Niels
AU - Voskuil, Michiel
AU - Schirmer, Stephan H.
AU - Ulusans, Susann
AU - Bode, Christoph
AU - Buschmann, Ivo R.
AU - Piek, Jan J.
PY - 2005
Y1 - 2005
N2 - Objective: Circulating leukocytes play a crucial role during arteriogenesis. However, known pro-arteriogenic compounds (MCP-1, GM-CSF) acting via monocytic pathways also exert positive effects on granulocytes and lymphocytes. The role of these two cell types in arteriogenesis remains yet to be clarified, which was the aim of the current study. Methods: Ninety New Zealand White Rabbits received either phosphate buffered saline (PBS), monocyte chemoattractant protein-1 (MCP-1), interleukin-8 (IL-8). neutrophil activating protein-2 (NAP-2) or lymphotactin (Ltn) via osmotic minipumps after unilateral femoral artery ligation. In vitro stimulation and in vivo assessment of chemoattraction confirmed cell-specific action of the compounds in rabbits. Arteriogenesis was evaluated by angiography and collateral conductance measurements using fluorescent microspheres. Quantitative immunohistology was used to quantify transmigrated leukocyte subtypes after infusion of the factors. Results: MCP-1 infusion attracts monocytes and granulocytes, whereas IL-8 attracts all three cell types albeit monocytes to a significantly lower degree than MCP-1. NAP-2 and lymphotactin selectively attract granulocytes, respectively, lymphocytes. Of the tested cytokines, only MCP-1 stimulates arteriogenesis, as assessed by collateral conductance measurements ((ml/(min 100 mm/Hg)): PBS, 50.70 +/- 5.15; MCP-1, 216.30 +/- 12.30: IL-8. 58.91 +/- 5.56; NAP-2, 66.83 +/- 8.72; Ltn, 52.80 +/- 5.37) and angiographic findings. Conclusion: This study for the first time provides evidence that not granulocytes or T-lymphocytes but monocytes are the key mediators of arteriogenesis. (c) 2005 Elsevier Ireland Ltd. All rights reserved
AB - Objective: Circulating leukocytes play a crucial role during arteriogenesis. However, known pro-arteriogenic compounds (MCP-1, GM-CSF) acting via monocytic pathways also exert positive effects on granulocytes and lymphocytes. The role of these two cell types in arteriogenesis remains yet to be clarified, which was the aim of the current study. Methods: Ninety New Zealand White Rabbits received either phosphate buffered saline (PBS), monocyte chemoattractant protein-1 (MCP-1), interleukin-8 (IL-8). neutrophil activating protein-2 (NAP-2) or lymphotactin (Ltn) via osmotic minipumps after unilateral femoral artery ligation. In vitro stimulation and in vivo assessment of chemoattraction confirmed cell-specific action of the compounds in rabbits. Arteriogenesis was evaluated by angiography and collateral conductance measurements using fluorescent microspheres. Quantitative immunohistology was used to quantify transmigrated leukocyte subtypes after infusion of the factors. Results: MCP-1 infusion attracts monocytes and granulocytes, whereas IL-8 attracts all three cell types albeit monocytes to a significantly lower degree than MCP-1. NAP-2 and lymphotactin selectively attract granulocytes, respectively, lymphocytes. Of the tested cytokines, only MCP-1 stimulates arteriogenesis, as assessed by collateral conductance measurements ((ml/(min 100 mm/Hg)): PBS, 50.70 +/- 5.15; MCP-1, 216.30 +/- 12.30: IL-8. 58.91 +/- 5.56; NAP-2, 66.83 +/- 8.72; Ltn, 52.80 +/- 5.37) and angiographic findings. Conclusion: This study for the first time provides evidence that not granulocytes or T-lymphocytes but monocytes are the key mediators of arteriogenesis. (c) 2005 Elsevier Ireland Ltd. All rights reserved
U2 - https://doi.org/10.1016/j.atherosclerosis.2005.01.047
DO - https://doi.org/10.1016/j.atherosclerosis.2005.01.047
M3 - Article
C2 - 16039282
SN - 0021-9150
VL - 181
SP - 285
EP - 293
JO - Atherosclerosis
JF - Atherosclerosis
IS - 2
ER -