Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).

Original languageEnglish
Article number102098
JournalSTAR Protocols
Issue number1
Publication statusPublished - 17 Mar 2023


  • Cancer
  • Cell Culture
  • Health Sciences
  • Microscopy
  • Model Organisms

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