@article{4cd6b566f4ba4a85a66200fc4f788001,
title = "Local irradiation of patient-derived tumors in immunodeficient mice",
abstract = "Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkdscid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).1",
keywords = "Cancer, Cell Culture, Health Sciences, Microscopy, Model Organisms",
author = "{van der Zalm}, {Amber P.} and Sanne Bootsma and Rodermond, {Hans M.} and Oei, {Arlene L.} and Bijlsma, {Maarten F.}",
note = "Funding Information: M.F.B. has received research funding from Celgene, Frame Therapeutics, and Lead Pharma and has acted as a consultant to Servier. Funding Information: This work was supported by KWF Dutch Cancer Society to M.F.B. (project number 10992 ). We would like to thank Cynthia Waasdorp, Marieke ten Brink, and the staff of the animal facility for technical assistance. We would like to acknowledge Eric van der Zalm and Gerbrand de Jong for creating the technical drawing and providing the files of the mice radiation setup. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = mar,
day = "17",
doi = "https://doi.org/10.1016/j.xpro.2023.102098",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",
}