TY - JOUR
T1 - Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients
AU - Laheij, Alexa M.G.A.
AU - Rozema, Frederik R.
AU - Brennan, Michael T.
AU - Bültzingslöwen, Inger von
AU - van Leeuwen, Stephanie J.M.
AU - Potting, Carin
AU - Huysmans, Marie Charlotte D.N.J.M.
AU - Hazenberg, Mette D.
AU - Brandt, Bernd W.
AU - Zaura, Egija
AU - Buijs, Mark J.
AU - de Soet, Johannes J.
AU - Blijlevens, Nicole N.M.
AU - Raber-Durlacher, Judith E.
N1 - Funding Information: This study was performed as an ancillary study of the Orastem study, an international, observational, prospective study into the impact of oral complications after stem cell transplantation [17]. This study was approved by the Medical Research Ethical Committee from Amsterdam University Medical Centers location AMC and the Radboud University Medical Center Nijmegen (NL52117.018.15) and is registered in the Netherlands Trial Register (NL5645). The study was funded by the Dutch Cancer Foundation (ACTA 2014-7468). The study was carried out in accordance with the relevant guidelines and regulations. All patients provided written informed consent. The data on the oral microbiome, salivary proteome, and oral mucositis in autologous SCT patients from the same study are reported elsewhere [10,18]. The demographic and other relevant data were retrieved from the medical charts. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Stem cell transplantation (SCT) is associated with oral microbial dysbiosis. However, long-term longitudinal data are lacking. Therefore, this study aimed to longitudinally assess the oral microbiome in SCT patients and to determine if changes are associated with oral mucositis and oral chronic graft-versus-host disease. Fifty allogeneic SCT recipients treated in two Dutch university hospitals were prospectively followed, starting at pre-SCT, weekly during hospitalization, and at 3, 6, 12, and 18 months after SCT. Oral rinsing samples were taken, and oral mucositis (WHO score) and oral chronic graft-versus-host disease (NIH score) were assessed. The oral microbiome diversity (Shannon index) and composition significantly changed after SCT and returned to pre-treatment levels from 3 months after SCT. Oral mucositis was associated with a more pronounced decrease in microbial diversity and with several disease-associated genera, such as Mycobacterium, Staphylococcus, and Enterococcus. On the other hand, microbiome diversity and composition were not associated with oral chronic graft-versus-host disease. To conclude, dysbiosis of the oral microbiome occurred directly after SCT but recovered after 3 months. Diversity and composition were related to oral mucositis but not to oral chronic graft-versus-host disease.
AB - Stem cell transplantation (SCT) is associated with oral microbial dysbiosis. However, long-term longitudinal data are lacking. Therefore, this study aimed to longitudinally assess the oral microbiome in SCT patients and to determine if changes are associated with oral mucositis and oral chronic graft-versus-host disease. Fifty allogeneic SCT recipients treated in two Dutch university hospitals were prospectively followed, starting at pre-SCT, weekly during hospitalization, and at 3, 6, 12, and 18 months after SCT. Oral rinsing samples were taken, and oral mucositis (WHO score) and oral chronic graft-versus-host disease (NIH score) were assessed. The oral microbiome diversity (Shannon index) and composition significantly changed after SCT and returned to pre-treatment levels from 3 months after SCT. Oral mucositis was associated with a more pronounced decrease in microbial diversity and with several disease-associated genera, such as Mycobacterium, Staphylococcus, and Enterococcus. On the other hand, microbiome diversity and composition were not associated with oral chronic graft-versus-host disease. To conclude, dysbiosis of the oral microbiome occurred directly after SCT but recovered after 3 months. Diversity and composition were related to oral mucositis but not to oral chronic graft-versus-host disease.
KW - allogeneic stem cell transplant
KW - conditioning
KW - dysbiosis
KW - oral graft-versus-host disease
KW - oral microbiome
KW - oral mucositis
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U2 - https://doi.org/10.3390/microorganisms10040734
DO - https://doi.org/10.3390/microorganisms10040734
M3 - Article
C2 - 35456787
SN - 2076-2607
VL - 10
SP - 1
EP - 13
JO - Microorganisms
JF - Microorganisms
IS - 4
M1 - 734
ER -