Lung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties

Valeria da Costa, Karina V. Mariño, Santiago A. Rodríguez-Zraquia, María Florencia Festari, Pablo Lores, Monique Costa, Mercedes Landeira, Gabriel A. Rabinovich, Sandra J. van Vliet, Teresa Freire

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Lung cancer is the first leading cause of cancer-related deaths in the world. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, consisting of N-acetyl-galactosamine (GalNAc) linked to a serine or threonine residue in proteins (α-GalNAc-O-Ser/Thr). The Tn antigen can be recognized by the Macrophage Galactose/GalNAc lectin (MGL), which mediates various immune regulatory and tolerogenic functions, mainly by reprogramming the maturation of function of dendritic cells (DCs). In this work, we generated two different Tn-expressing variants from the Lewis-type lung murine cancer cell line LL/2, which showed different alterations in the O-glycosylation pathways that influenced the interaction with mouse MGL2 and the immunomodulatory properties of DCs. Thus, the identification of the biological programs triggered by Tn+ cancer cells might contribute to an improved understanding of the molecular mechanisms elicited by MGL-dependent immune regulatory circuits.
Original languageEnglish
Article number12047
JournalInternational journal of molecular sciences
Issue number19
Publication statusPublished - 1 Oct 2022


  • Macrophage Galactose-type lectin
  • O-glycosylation
  • Tn antigen
  • dendritic cells
  • lung cancer

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