Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA

R. Benne; J. van den Burg; J. P. Brakenhoff; P. Sloof; J. H. van Boom; M. C. Tromp

doi:https://doi.org/10.1016/0092-8674(86)90063-2

Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA

R. Benne, J. van den Burg, J. P. Brakenhoff, P. Sloof, J. H. van Boom, M. C. Tromp

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Abstract

The mitochondrial cytochrome oxidase (cox) subunit II gene from trypanosomes contains a frameshift at amino acid 170. This gene is highly conserved in different trypanosome species, suggesting that it is functional. Sequence determination of coxII transcripts of T. brucei and C. fasciculata reveals four extra, reading frame-restoring nucleotides at the frameshift position that are not encoded in the DNA. Southern blot analysis of DNA of both trypanosome species failed to show the existence of a second version of the coxII gene. We conclude, therefore, that the extra nucleotides are added during or after transcription of the frameshift gene by an RNA-editing process

Original language	English
Pages (from-to)	819-826
Journal	Cell
Volume	46
Issue number	6
DOIs	https://doi.org/10.1016/0092-8674(86)90063-2
Publication status	Published - 1986

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https://doi.org/10.1016/0092-8674(86)90063-2

Cite this

@article{80426fd10ba141798c5ac709736473e2,

title = "Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA",

abstract = "The mitochondrial cytochrome oxidase (cox) subunit II gene from trypanosomes contains a frameshift at amino acid 170. This gene is highly conserved in different trypanosome species, suggesting that it is functional. Sequence determination of coxII transcripts of T. brucei and C. fasciculata reveals four extra, reading frame-restoring nucleotides at the frameshift position that are not encoded in the DNA. Southern blot analysis of DNA of both trypanosome species failed to show the existence of a second version of the coxII gene. We conclude, therefore, that the extra nucleotides are added during or after transcription of the frameshift gene by an RNA-editing process",

author = "R. Benne and {van den Burg}, J. and Brakenhoff, {J. P.} and P. Sloof and {van Boom}, {J. H.} and Tromp, {M. C.}",

year = "1986",

doi = "https://doi.org/10.1016/0092-8674(86)90063-2",

language = "English",

volume = "46",

pages = "819--826",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "6",

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TY - JOUR

T1 - Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA

AU - Benne, R.

AU - van den Burg, J.

AU - Brakenhoff, J. P.

AU - Sloof, P.

AU - van Boom, J. H.

AU - Tromp, M. C.

PY - 1986

Y1 - 1986

N2 - The mitochondrial cytochrome oxidase (cox) subunit II gene from trypanosomes contains a frameshift at amino acid 170. This gene is highly conserved in different trypanosome species, suggesting that it is functional. Sequence determination of coxII transcripts of T. brucei and C. fasciculata reveals four extra, reading frame-restoring nucleotides at the frameshift position that are not encoded in the DNA. Southern blot analysis of DNA of both trypanosome species failed to show the existence of a second version of the coxII gene. We conclude, therefore, that the extra nucleotides are added during or after transcription of the frameshift gene by an RNA-editing process

AB - The mitochondrial cytochrome oxidase (cox) subunit II gene from trypanosomes contains a frameshift at amino acid 170. This gene is highly conserved in different trypanosome species, suggesting that it is functional. Sequence determination of coxII transcripts of T. brucei and C. fasciculata reveals four extra, reading frame-restoring nucleotides at the frameshift position that are not encoded in the DNA. Southern blot analysis of DNA of both trypanosome species failed to show the existence of a second version of the coxII gene. We conclude, therefore, that the extra nucleotides are added during or after transcription of the frameshift gene by an RNA-editing process

U2 - https://doi.org/10.1016/0092-8674(86)90063-2

DO - https://doi.org/10.1016/0092-8674(86)90063-2

M3 - Article

C2 - 3019552

SN - 0092-8674

VL - 46

SP - 819

EP - 826

JO - Cell

JF - Cell

IS - 6

ER -