Memory CD8+ T cells upregulate glycolysis and effector functions under limiting oxygen conditions

Ammarina Beumer-Chuwonpad, Floris P. J. van Alphen, Natasja A. M. Kragten, Julian J. Freen-van Heeren, Maria Rodriguez Gomez, Arthur J. Verhoeven, Maartje van den Biggelaar, Klaas P. J. M. van Gisbergen

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Memory CD8+ T cells are indispensable for maintaining long-term immunity against intracellular pathogens and tumors. Despite their presence at oxygen-deprived infected tissue sites or in tumors, the impact of local oxygen pressure on memory CD8+ T cells remains largely unclear. We sought to elucidate how oxygen pressure impacts memory CD8+ T cells arising after infection with Listeria monocytogenes-OVA. Our data revealed that reduced oxygen pressure during in vitro culture switched CD8+ T cell metabolism from oxidative phosphorylation to a glycolytic phenotype. Quantitative proteomic analysis showed that limiting oxygen conditions increased the expression of glucose transporters and components of the glycolytic pathway, while decreasing TCA cycle and mitochondrial respiratory chain proteins. The altered CD8+ T cell metabolism did not affect the expansion potential, but enhanced the granzyme B and IFN-γ production capacity. In vivo, memory CD8+ T cells cultured under low oxygen pressure provided protection against bacterial rechallenge. Taken together, our study indicates that strategies of cellular immune therapy may benefit from reducing oxygen during culture to develop memory CD8+ T cells with superior effector functions.
Original languageEnglish
Article number2249918
Number of pages16
JournalEuropean journal of immunology
Volume53
Issue number2
Early online date2022
DOIs
Publication statusPublished - Feb 2023

Keywords

  • CD8 memory T cells
  • Cytotoxic T cells
  • Hypoxic T cell cultures
  • T cell metabolism
  • glycolysis

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