TY - JOUR
T1 - Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation
AU - Molenaars, Marte
AU - Schomakers, Bauke V.
AU - Elfrink, Hyung L.
AU - Gao, Arwen W.
AU - Vervaart, Martin A. T.
AU - Pras-Raves, Mia L.
AU - Luyf, Angela C.
AU - Smith, Reuben L.
AU - Sterken, Mark G.
AU - Kammenga, Jan E.
AU - van Kampen, Antoine H. C.
AU - Janssens, Georges E.
AU - Vaz, Frédéric M.
AU - van Weeghel, Michel
AU - Houtkooper, Riekelt H.
N1 - Funding Information: Work in the Houtkooper group is financially supported by a European Research Council Starting grant (no. 638290), a VIDI grant from ZonMw (no. 91715305) and a grant from Velux Stiftung (no. 1063). Publisher Copyright: © 2021. Published by The Company of Biologists Ltd.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand; for instance, in research related to nutrition and aging. The nematode Caenorhabditis elegans is a key model organism in these fields, owing to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single-sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for example, glycolysis and the tricarboxylic acid cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.
AB - Comprehensive metabolomic and lipidomic mass spectrometry methods are in increasing demand; for instance, in research related to nutrition and aging. The nematode Caenorhabditis elegans is a key model organism in these fields, owing to the large repository of available C. elegans mutants and their convenient natural lifespan. Here, we describe a robust and sensitive analytical method for the semi-quantitative analysis of >100 polar (metabolomics) and >1000 apolar (lipidomics) metabolites in C. elegans, using a single-sample preparation. Our method is capable of reliably detecting a wide variety of biologically relevant metabolic aberrations in, for example, glycolysis and the tricarboxylic acid cycle, pyrimidine metabolism and complex lipid biosynthesis. In conclusion, we provide a powerful analytical tool that maximizes metabolic data yield from a single sample.
KW - C. elegans
KW - Lipidomics
KW - Metabolism
KW - Metabolomics
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85105410165&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/33653825
U2 - https://doi.org/10.1242/dmm.047746
DO - https://doi.org/10.1242/dmm.047746
M3 - Article
C2 - 33653825
SN - 1754-8403
VL - 14
JO - Disease models & mechanisms
JF - Disease models & mechanisms
IS - 4
M1 - dmm.047746
ER -