Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and De Novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labeled Docosanoic Acid

Malu-Clair van de Beek, Inge M. E. Dijkstra, Stephan Kemp

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

Peroxisomes are present in virtually every eukaryotic cell type with the exception of the mature erythrocyte. In higher eukaryotes, one of the main functions of peroxisomes is lipid metabolism by means of beta-oxidation of very long-chain fatty acids (VLCFA; ≥22 carbon atoms). A dysfunction in peroxisomal VLCFA beta-oxidation results in elevated VLCFA levels in cells, tissue, and plasma. Here, we describe a straightforward and sensitive method to measure peroxisomal beta-oxidation capacity in living cells using stable-isotope labeled docosanoic acid (D3-C22:0)
Original languageEnglish
Pages (from-to)45-54
JournalMethods in molecular biology (Clifton, N.J.)
Volume1595
DOIs
Publication statusPublished - 2017

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