Methodological Guidelines to Study Extracellular Vesicles

Frank A W Coumans; Alain R Brisson; Edit I Buzas; Françoise Dignat-George; Esther E E Drees; Samir El-Andaloussi; Costanza Emanueli; Aleksandra Gasecka; An Hendrix; Andrew F Hill; Romaric Lacroix; Yi Lee; Ton G van Leeuwen; Nigel Mackman; Imre Mäger; John P Nolan; Edwin van der Pol; D Michiel Pegtel; Susmita Sahoo; Pia R M Siljander; Guus Sturk; Olivier de Wever; Rienk Nieuwland

doi:https://doi.org/10.1161/CIRCRESAHA.117.309417

Methodological Guidelines to Study Extracellular Vesicles

Frank A W Coumans, Alain R Brisson, Edit I Buzas, Françoise Dignat-George, Esther E E Drees, Samir El-Andaloussi, Costanza Emanueli, Aleksandra Gasecka, An Hendrix, Andrew F Hill, Romaric Lacroix, Yi Lee, Ton G van Leeuwen, Nigel Mackman, Imre Mäger, John P Nolan, Edwin van der Pol, D Michiel Pegtel, Susmita Sahoo, Pia R M SiljanderGuus Sturk, Olivier de Wever, Rienk Nieuwland

Research output: Contribution to journal › Review article › Academic › peer-review

680 Citations (Scopus)

Abstract

Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

Original language	English
Pages (from-to)	1632-1648
Number of pages	17
Journal	Circulation Research
Volume	120
Issue number	10
DOIs	https://doi.org/10.1161/CIRCRESAHA.117.309417
Publication status	Published - 12 May 2017

Keywords

Biomarkers
Blood Specimen Collection
Cardiovascular Diseases
Exosomes
Extracellular Vesicles
Flow Cytometry
Humans
Journal Article
Review

Access to Document

https://doi.org/10.1161/CIRCRESAHA.117.309417

Cite this

Coumans, F. A. W., Brisson, A. R., Buzas, E. I., Dignat-George, F., Drees, E. E. E., El-Andaloussi, S., Emanueli, C., Gasecka, A., Hendrix, A., Hill, A. F., Lacroix, R., Lee, Y., van Leeuwen, T. G., Mackman, N., Mäger, I., Nolan, J. P., van der Pol, E., Pegtel, D. M., Sahoo, S., ... Nieuwland, R. (2017). Methodological Guidelines to Study Extracellular Vesicles. Circulation Research, 120(10), 1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417

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title = "Methodological Guidelines to Study Extracellular Vesicles",

abstract = "Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.",

keywords = "Biomarkers, Blood Specimen Collection, Cardiovascular Diseases, Exosomes, Extracellular Vesicles, Flow Cytometry, Humans, Journal Article, Review",

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year = "2017",

month = may,

day = "12",

doi = "https://doi.org/10.1161/CIRCRESAHA.117.309417",

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Coumans, FAW, Brisson, AR, Buzas, EI, Dignat-George, F, Drees, EEE, El-Andaloussi, S, Emanueli, C, Gasecka, A, Hendrix, A, Hill, AF, Lacroix, R, Lee, Y, van Leeuwen, TG, Mackman, N, Mäger, I, Nolan, JP, van der Pol, E , Pegtel, DM, Sahoo, S, Siljander, PRM, Sturk, G, de Wever, O & Nieuwland, R 2017, 'Methodological Guidelines to Study Extracellular Vesicles', Circulation Research, vol. 120, no. 10, pp. 1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417

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T1 - Methodological Guidelines to Study Extracellular Vesicles

AU - Coumans, Frank A W

AU - Brisson, Alain R

AU - Buzas, Edit I

AU - Dignat-George, Françoise

AU - Drees, Esther E E

AU - El-Andaloussi, Samir

AU - Emanueli, Costanza

AU - Gasecka, Aleksandra

AU - Hendrix, An

AU - Hill, Andrew F

AU - Lacroix, Romaric

AU - Lee, Yi

AU - van Leeuwen, Ton G

AU - Mackman, Nigel

AU - Mäger, Imre

AU - Nolan, John P

AU - van der Pol, Edwin

AU - Pegtel, D Michiel

AU - Sahoo, Susmita

AU - Siljander, Pia R M

AU - Sturk, Guus

AU - de Wever, Olivier

AU - Nieuwland, Rienk

PY - 2017/5/12

Y1 - 2017/5/12

N2 - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

AB - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

KW - Biomarkers

KW - Blood Specimen Collection

KW - Cardiovascular Diseases

KW - Exosomes

KW - Extracellular Vesicles

KW - Flow Cytometry

KW - Humans

KW - Journal Article

KW - Review

U2 - https://doi.org/10.1161/CIRCRESAHA.117.309417

DO - https://doi.org/10.1161/CIRCRESAHA.117.309417

M3 - Review article

C2 - 28495994

SN - 0009-7330

VL - 120

SP - 1632

EP - 1648

JO - Circulation Research

JF - Circulation Research

IS - 10

ER -