Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

Rebekka M. Koeck; Florence Busato; Jorg Tost; Dimitri Consten; Jannie van Echten-Arends; Sebastiaan Mastenbroek; Yvonne Wurth; Sylvie Remy; Sabine Langie; Tim S. Nawrot; Michelle Plusquin; Rossella Alfano; Esmée M. Bijnens; Marij Gielen; Ron van Golde; John C. M. Dumoulin; Han Brunner; Aafke P. A. van Montfoort; Masoud Zamani Esteki

doi:https://doi.org/10.1038/s41525-022-00310-3

Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

Rebekka M. Koeck, Florence Busato, Jorg Tost, Dimitri Consten, Jannie van Echten-Arends, Sebastiaan Mastenbroek, Yvonne Wurth, Sylvie Remy, Sabine Langie, Tim S. Nawrot, Michelle Plusquin, Rossella Alfano, Esmée M. Bijnens, Marij Gielen, Ron van Golde, John C. M. Dumoulin, Han Brunner, Aafke P. A. van Montfoort, Masoud Zamani Esteki

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.

Original language	English
Article number	39
Journal	NPJ GENOMIC MEDICINE
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41525-022-00310-3
Publication status	Published - Dec 2022

Access to Document

https://doi.org/10.1038/s41525-022-00310-3

Cite this

Koeck, R. M., Busato, F., Tost, J., Consten, D., van Echten-Arends, J., Mastenbroek, S., Wurth, Y., Remy, S., Langie, S., Nawrot, T. S., Plusquin, M., Alfano, R., Bijnens, E. M., Gielen, M., van Golde, R., Dumoulin, J. C. M., Brunner, H., van Montfoort, A. P. A., & Zamani Esteki, M. (2022). Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences. NPJ GENOMIC MEDICINE, 7(1), [39]. https://doi.org/10.1038/s41525-022-00310-3

@article{c210e37cd4554243a22fd7066afb9a88,

title = "Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences",

abstract = "A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.",

author = "Koeck, {Rebekka M.} and Florence Busato and Jorg Tost and Dimitri Consten and {van Echten-Arends}, Jannie and Sebastiaan Mastenbroek and Yvonne Wurth and Sylvie Remy and Sabine Langie and Nawrot, {Tim S.} and Michelle Plusquin and Rossella Alfano and Bijnens, {Esm{\'e}e M.} and Marij Gielen and {van Golde}, Ron and Dumoulin, {John C. M.} and Han Brunner and {van Montfoort}, {Aafke P. A.} and {Zamani Esteki}, Masoud",

note = "Funding Information: We thank the IVF couples who have agreed for their children to participate in this study. We thank all the employees of the IVF clinics and obstetrics departments involved in recruiting participants and collecting samples for this study. We thank E. Pishva for his consultation regarding data analysis and for the critical reading of the manuscript. This study was funded by March of Dimes (6-FY13-153). It was further supported by the stichting fertility foundation, EVA (Erfelijkheid Voortplanting & Aanleg) speciality programme (grant no. KP111513) of Maastricht University Medical Centre (MUMC+), and the Horizon 2020 innovation (ERIN) (grant no. EU952516) of the European Commission. We thank the FLEHS Supervisory Board for the provision of data. The FLEHS studies are commissioned, financed, and steered by the Flemish Government (Department of Economy, Science and Innovations, Agency for Care and Health and Department of Environment). The FLEHS dataset has been generated by the Flemish Center of Expertise on Environment and Health (FLEHS 2016–2020), funded by the Environment, Nature and Energy Department of the Flemish government. The views expressed in this manuscript are those of the author(s) and are not necessarily endorsed by the Flemish government. Funding Information: We thank the IVF couples who have agreed for their children to participate in this study. We thank all the employees of the IVF clinics and obstetrics departments involved in recruiting participants and collecting samples for this study. We thank E. Pishva for his consultation regarding data analysis and for the critical reading of the manuscript. This study was funded by March of Dimes (6-FY13-153). It was further supported by the stichting fertility foundation, EVA (Erfelijkheid Voortplanting & Aanleg) speciality programme (grant no. KP111513) of Maastricht University Medical Centre (MUMC+), and the Horizon 2020 innovation (ERIN) (grant no. EU952516) of the European Commission. We thank the FLEHS Supervisory Board for the provision of data. The FLEHS studies are commissioned, financed, and steered by the Flemish Government (Department of Economy, Science and Innovations, Agency for Care and Health and Department of Environment). The FLEHS dataset has been generated by the Flemish Center of Expertise on Environment and Health (FLEHS 2016–2020), funded by the Environment, Nature and Energy Department of the Flemish government. The views expressed in this manuscript are those of the author(s) and are not necessarily endorsed by the Flemish government. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "https://doi.org/10.1038/s41525-022-00310-3",

language = "English",

volume = "7",

journal = "npj Genomic Medicine",

issn = "2056-7944",

publisher = "Nature Publishing Group",

number = "1",

}

Koeck, RM, Busato, F, Tost, J, Consten, D, van Echten-Arends, J, Mastenbroek, S, Wurth, Y, Remy, S, Langie, S, Nawrot, TS, Plusquin, M, Alfano, R, Bijnens, EM, Gielen, M, van Golde, R, Dumoulin, JCM, Brunner, H, van Montfoort, APA & Zamani Esteki, M 2022, 'Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences', NPJ GENOMIC MEDICINE, vol. 7, no. 1, 39. https://doi.org/10.1038/s41525-022-00310-3

TY - JOUR

T1 - Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

AU - Koeck, Rebekka M.

AU - Busato, Florence

AU - Tost, Jorg

AU - Consten, Dimitri

AU - van Echten-Arends, Jannie

AU - Mastenbroek, Sebastiaan

AU - Wurth, Yvonne

AU - Remy, Sylvie

AU - Langie, Sabine

AU - Nawrot, Tim S.

AU - Plusquin, Michelle

AU - Alfano, Rossella

AU - Bijnens, Esmée M.

AU - Gielen, Marij

AU - van Golde, Ron

AU - Dumoulin, John C. M.

AU - Brunner, Han

AU - van Montfoort, Aafke P. A.

AU - Zamani Esteki, Masoud

N1 - Funding Information: We thank the IVF couples who have agreed for their children to participate in this study. We thank all the employees of the IVF clinics and obstetrics departments involved in recruiting participants and collecting samples for this study. We thank E. Pishva for his consultation regarding data analysis and for the critical reading of the manuscript. This study was funded by March of Dimes (6-FY13-153). It was further supported by the stichting fertility foundation, EVA (Erfelijkheid Voortplanting & Aanleg) speciality programme (grant no. KP111513) of Maastricht University Medical Centre (MUMC+), and the Horizon 2020 innovation (ERIN) (grant no. EU952516) of the European Commission. We thank the FLEHS Supervisory Board for the provision of data. The FLEHS studies are commissioned, financed, and steered by the Flemish Government (Department of Economy, Science and Innovations, Agency for Care and Health and Department of Environment). The FLEHS dataset has been generated by the Flemish Center of Expertise on Environment and Health (FLEHS 2016–2020), funded by the Environment, Nature and Energy Department of the Flemish government. The views expressed in this manuscript are those of the author(s) and are not necessarily endorsed by the Flemish government. Funding Information: We thank the IVF couples who have agreed for their children to participate in this study. We thank all the employees of the IVF clinics and obstetrics departments involved in recruiting participants and collecting samples for this study. We thank E. Pishva for his consultation regarding data analysis and for the critical reading of the manuscript. This study was funded by March of Dimes (6-FY13-153). It was further supported by the stichting fertility foundation, EVA (Erfelijkheid Voortplanting & Aanleg) speciality programme (grant no. KP111513) of Maastricht University Medical Centre (MUMC+), and the Horizon 2020 innovation (ERIN) (grant no. EU952516) of the European Commission. We thank the FLEHS Supervisory Board for the provision of data. The FLEHS studies are commissioned, financed, and steered by the Flemish Government (Department of Economy, Science and Innovations, Agency for Care and Health and Department of Environment). The FLEHS dataset has been generated by the Flemish Center of Expertise on Environment and Health (FLEHS 2016–2020), funded by the Environment, Nature and Energy Department of the Flemish government. The views expressed in this manuscript are those of the author(s) and are not necessarily endorsed by the Flemish government. Publisher Copyright: © 2022, The Author(s).

PY - 2022/12

Y1 - 2022/12

N2 - A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.

AB - A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.

UR - http://www.scopus.com/inward/record.url?scp=85133104338&partnerID=8YFLogxK

U2 - https://doi.org/10.1038/s41525-022-00310-3

DO - https://doi.org/10.1038/s41525-022-00310-3

M3 - Article

C2 - 35768464

VL - 7

JO - npj Genomic Medicine

JF - npj Genomic Medicine

SN - 2056-7944

IS - 1

M1 - 39

ER -

Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

Abstract

Access to Document

Other files and links

Cite this