Microfluidic protocol for in vitro culture of human embryos

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

In vitro culture of pre-implantation embryos is a key-step in Assisted Reproductive Technologies (ART) protocols. In present work we examine the potential of microfluidic devices for pre-implantation human embryo culture, in comparison with conventional droplet-based culture (control). Donated frozen day-4 embryos are cultured for 72 h after thawing in both formats, and graded at four different time points (24, 28, 48 & 72 h). Specifically, pre-implantation developmental rates (blastocyst rates) and embryo qualities are evaluated. Blastocyst rates show no statistical difference between the two culture groups, while the embryo distribution shifts towards higher quality blastocysts in the microfluidic group.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages536-538
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 27 Oct 201331 Oct 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period27/10/201331/10/2013

Keywords

  • Blastocyst rate
  • Embryo quality
  • Human embryo culture
  • Microfluidics
  • Prospective randomized controlled trial

Cite this