TY - JOUR
T1 - Minimum information to report about a flow cytometry experiment on extracellular vesicles
T2 - Communication from the ISTH SSC subcommittee on vascular biology
AU - van der Pol, Edwin
AU - Welsh, Joshua A.
AU - Nieuwland, Rienk
N1 - Funding Information: EvdP is funded by the Dutch Research Council (NWO), Grant/Award Number: VENI 15924. JAW is funded by the National Institutes of Health (NIH), Grant/Award Numbers: UG3 TR002881 and ZIA BC011502. RN is funded by the European Association of National Metrology Institutes, Grant/Award Number: 18HLT01 METVES II. Publisher Copyright: © 2021 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
PY - 2022/1
Y1 - 2022/1
N2 - The Extracellular Vesicle Flow Cytometry Working Group (http://www.evflowcytometry.org) is formed by members of the International Society for Extracellular Vesicles (ISEV), the International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH). This working group of flow cytometry experts develops guidelines for best practices regarding flow cytometry detection of extracellular vesicles. To improve rigor and standardization, this working group published a framework outlining the minimal information to report about a flow cytometry experiment on extracellular vesicles (MIFlowCyt-EV) in the Journal of Extracellular Vesicles, the ISEV journal, in 2020. In parallel, an article explaining MIFlowCyt-EV was published in Cytometry Part A, one of the ISAC journals, and now will be introduced to the ISTH as an SSC Communication in the Journal of Thrombosis and Haemostasis. The goal of this SSC Communication is to explain why flow cytometry is becoming the instrument of choice to characterize single extracellular vesicles, the obstacles that have been identified and (mostly) overcome by developing procedures to calibrate flow cytometers, and the relevance of reporting minimal information to improve reliability and reproducibility of experiments in which flow cytometers are used for characterization of extracellular vesicles.
AB - The Extracellular Vesicle Flow Cytometry Working Group (http://www.evflowcytometry.org) is formed by members of the International Society for Extracellular Vesicles (ISEV), the International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH). This working group of flow cytometry experts develops guidelines for best practices regarding flow cytometry detection of extracellular vesicles. To improve rigor and standardization, this working group published a framework outlining the minimal information to report about a flow cytometry experiment on extracellular vesicles (MIFlowCyt-EV) in the Journal of Extracellular Vesicles, the ISEV journal, in 2020. In parallel, an article explaining MIFlowCyt-EV was published in Cytometry Part A, one of the ISAC journals, and now will be introduced to the ISTH as an SSC Communication in the Journal of Thrombosis and Haemostasis. The goal of this SSC Communication is to explain why flow cytometry is becoming the instrument of choice to characterize single extracellular vesicles, the obstacles that have been identified and (mostly) overcome by developing procedures to calibrate flow cytometers, and the relevance of reporting minimal information to improve reliability and reproducibility of experiments in which flow cytometers are used for characterization of extracellular vesicles.
KW - calibration
KW - extracellular vesicles
KW - flow cytometry
KW - reproducibility of results
KW - research design
UR - http://www.scopus.com/inward/record.url?scp=85119975667&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/jth.15540
DO - https://doi.org/10.1111/jth.15540
M3 - Article
C2 - 34637195
SN - 1538-7933
VL - 20
SP - 245
EP - 251
JO - Journal of thrombosis and haemostasis
JF - Journal of thrombosis and haemostasis
IS - 1
ER -