Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract

Vichununt Kerdput, Kritsakorn Kanjanapongkul, Arunporn Itharat, Ratchadaporn Pramong, Wouter H. Lamers, Theodorus B. M. Hakvoort, Aldo Jongejan, Wisuit Pradidarcheep

Research output: Contribution to journalArticleAcademicpeer-review


Hepatocellular carcinoma (HCC) is a primary liver cancer commonly found in adults. Previously, we showed the anticancer effects of Thai herbal plant extract, Dioscorea membranacea Pierre (DM), in HCC-bearing rats. In the present study, we further examined the proposed mechanism of DM, including apoptosis and antioxidant activity. Moreover, we used RNA sequencing (RNA-seq) to analyze molecular pathways in the rat model in which HCC was induced by diethylnitrosamine (DEN) and thioacetamide (TAA). The HCC-bearing rats were then treated with 40 mg/kg of DM for 8 weeks, after which experimental and control rats were sacrificed and liver tissues were collected. The RNA-seq data of DEN/TAA-treated rats exhibited upregulation of 16 hallmark pathways, including epithelial mesenchymal transition, inflammatory responses, and angiogenesis (p<0.01). DM extract expanded the Bax protein-positive pericentral zone in the tumor areas and decreased hepatic malondialdehyde levels, implying a decrease in lipid peroxidation in liver. However, DM treatment did not ameliorate the molecular pathways induced in DEN/TAA-treated livers. Our findings indicate that DM extract has antioxidant activity and exerts its pro-apoptotic effect on rat HCCs in vivo at the (post-)translational level.

Original languageEnglish
Pages (from-to)1806-1815
Number of pages10
JournalInternational Journal of Medical Sciences
Issue number12
Publication statusPublished - 2022


  • Dioscorea membranacea
  • RNA sequencing
  • apoptosis
  • liver cancer
  • malondialdehyde

Cite this